春萝卜雄性不育系4-05A小孢子败育的细胞学观察

Cytological Study on Anther Development of Spring Radish of Male Sterility Line 4-05A

为了确定其花粉败育的时期和细胞学特点,采用石蜡切片法,对春萝卜雄性不育系4-05A及其保持系4-05B小孢子发生和花粉发育过程进行观察、比较。结果表明:不育系和保持系小孢子母细胞都能正常进行减数分裂,四分体可以释放出小孢子,不育系4-05A败育发生在单核靠边期,解体后的绒毡层原生质团急剧而彻底地进入花粉囊,渗入花粉母细胞周围,并与花粉母细胞黏连在一起。

Observation of anther in spring Raphanussativus L.male sterility line（4-05A） and maintainer line（4-05B） was made by means of paraffin section method in order to determine the time that the anther development was inhibited and the sterile mode.The results showed that the meiosis of the connective of the sterile line and it′s maintainer line are normal,Tetrad can yield microspores,the sterile line（4-05A） Degenerated at Uninucleus microspore at periphery,The disintegrated tapetum cells infiltrated into the location around the pollen mother cells and conglutinated with the pollen mother cells.