摘要
从牛瘤胃内容物中筛选出一株兼性厌氧纤维素酶产生菌CR-14,根据形态特征、生理生化特征和16 S rDNA序列分析鉴定为纤维单胞菌(Celloulonmonas sp.).该菌株所产纤维素酶中主要组分酶包括C1酶、Cx酶和β-葡萄糖苷酶,其中Cx酶酶活最高,C1酶和β-葡萄糖苷酶酶活较低.该酶的最适反应条件为温度50℃、pH 6.0;在60℃以下、pH 6.0~7.5条件下性能稳定;Mn2+、Fe2+能显著促进该酶的活力,Zn2+、Cu2+、Ca2+、K+对酶活力有抑制作用.
Strain CR-14 producing extacellular cellulase was isolated from contents of cattal rumen.According to its morphological,the physiological and biochemical properties,16 S rDNA phylogenetic analysis,indicated that the strain belonged to Celloulonmonas sp.The main components of cellulase of strain CR-14 was CX,C1 and β-glucosidase.The activity of Cx was the highest,while activity of C1 and β-glucosidase was relatively low.The optimal enzyme reaction conditions was 50℃,pH 6.0.Under the conditions of 60℃ and pH 6.0-7.5 cellulase was stability.Mn2+,Fe2+ could significantly promote the vitality of the enzyme.Zn2+,Cu2+,Ca2+and K+ inhibit the vitality of the enzyme.
出处
《华北农学报》
CSCD
北大核心
2010年第6期139-143,共5页
Acta Agriculturae Boreali-Sinica
基金
国家科技支撑项目(2008BADCC4B02-8)
河北省科技支撑项目(08225502D)
关键词
牛瘤胃
兼性厌氧
菌种鉴定
纤维单胞菌
Cattal rumen
Facultative anaerobic
Identification
Celloulonmonas