麦麸膳食纤维的研制

Preparation of Dietary Fiber from Wheat Bran

采用生物酶法从麦麸中提取膳食纤维,通过水洗去除麦麸中的部分淀粉和蛋白质,在pH5.5、55℃条件下利用内源植酸酶去除麦麸中的植酸,水解同时加入中温α-淀粉酶与蛋白酶(Alcalase2.4L)来去除麦麸中淀粉与蛋白质。通过响应面优化麦麸膳食纤维制备工艺,得出最佳制备工艺条件为:pH7.0,蛋白酶添加量1.8%(4.32 AU/100 g麦麸),中温α-淀粉酶添加量1.72%(3 440 U/100 g麦麸),反应温度68℃,反应时间2.5 h。

The biological enzyme was used to extract dietary fiber from wheat bran.Partial starch and protein were removed by water washing from wheat bran.Phytic acid was removed with endogene phytase under pH5.5 and 55℃ conditions.The warm alpha-amylase and the proteinase（Alcalase2.4L） were used to remove starch and protein with the enzyme hydrolysis simultaneously.Preparation of dietary fiber from wheat bran was optimized with response surface analysis.The optimum conditions were as follows： pH7.0,proteinase 1.8%（4.32 AU/100 g wheat bran）,alpha-amylase 1.72%（3 440 U/100 g wheat bran）,temperature 68℃,time 2.5 h.