摘要
显微分离出两条黑麦B染色体,经蛋白酶K处理,Sau3AI酶切后,用LA-PCR(LinkerAdapterPCR)方法进行扩增,得到0.2~2kb的DNA片段.Southern杂交分析结果表明,此扩增产物来源于黑麦B染色体.将部分PCR产物克隆到pUC19载体中,获得了5000个克隆.
Two B chromosomes isolated from a metaphase
cell of rye by microdissection, were digested with proteinase K and Sau3AⅠ, then the B
chromosome DNA was amplified by LA PCR(Linker Adapter PCR).The PCR products are a smear
ranged from 0.2 kb to 2 kb. The Southern hybridization showed that the PCR products were
derived from the B chromosome of rye. Part of these PCR products were cloned into pUC19 and
we obtained 5000 clones. This microdissection and microcloning method is a simple and
general approach for constructing B chromosome DNA library.
出处
《南开大学学报(自然科学版)》
CAS
CSCD
北大核心
1999年第2期103-106,共4页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家自然科学基金
关键词
黑麦
B染色体
显微切割
微克隆
Secale cereale
B
chromosome
microdissection
microcloning