藻类富集金的机理初探

PRELIMINARY SURVEY OF MECHANISM OF ALGAES ENRICHING OF AU

通过对蓝藻、绿藻、隐藻三大门类的某些种的富集金模拟实验，确证了藻类富集金的普遍性．将藻类细胞的细胞壁（细胞膜）及一些细胞质膜从细胞中分离、提取出来，并测定了壁（膜）中金的含量；通过与整个细胞的金含量之间的对比。

In this research essay, we have simulated the experiments of the phenomenon of the Algaes collecting Au from circumstances in which lives on the base of the senior studies, and obstained a lot of new and defined conclusions. We can see a lot of reference data about the low organisms' ability of enriching gold in some earlier scientific works. But almost all of them only described the facts and some phenomena of algae's capability of enrichment. little referent information was related to the mechanism of microorganism's enrichment of metal elment. In order to clarify the mechanism of algaes enrichment of Au, we designed a series of schemes to simulate the metal enrichment conditions and process. Besides the blue green algae, which is recognized as the initial living things in early phase of the earth and its photosynthesis is the most important factor to bring today's earth a green, flourishing and prosperous landscape able to absorb Au from the environments, nearly all species of the other divisions agae also draw Au and store it up in certain position of their cell bodies. For example, some species of Cholophyta and Cryptophyta are able to do so. at first, we cultured various kinds of algae including some species of Cholophyta, Cryptophyta and Cyanophyta in evry diffirent conditions(including temperature, lighting intensity, pH and so on) and observed and studied which species were the best samples for our target of experiment. Then we chose some suitable ones [ Spirulina Platensis and Maxima (cyanophyta), Dunaliella Salina (Cholophyta), Cryptomanos sp. (Cryptophyta) ] and stared to culture them in designed concentrations of Au 3+ (aquatic state), and after some days, we harvested the ripe alage. However, to break the verytiny alga cell's wall(some kinds of algae have nearly no wall, only cell membrane for us to deal with easily) and extract it from the mixed broken cell solution was a difficult and key step of all experiments we ever did. Then we fathomed the Au content in both cellwall and the whole algae cell; by comparing with each other, we found that Au content in cell wall or membrane was higher than that in the whole cell sample; 80%～90% of the enriched Au of algae body was detected in cellwasll(membrane). So we have acquired that most of the enriched Au is absorbed in cell's wall and membrane and it isn't correct to think the enriched gold is distributed evenly in cell body.