摘要
目的探讨原发性高血压脑出血后,安宫牛黄丸对血肿周围组织细胞凋亡与核转录因子-κB(NF-κB)的表达、NO含量的干预作用。方法采用自体血注入法制作脑出血模型,分为模型组、中药组、西药组、中西药结合组进行干预治疗研究,采用免疫组化方法测定NF-κB的表达,并测定血清中NO含量。结果中西药结合组、中药组、西药组NF-κB的表达明显高于模型组(P<0.05),各治疗组之间无统计学差异(P>0.05)。中西药结合组血NO含量明显低于其他三组(P<0.05),中西药结合组、中药组、西药组脑组织血NO含量均低于模型组(P<0.05),中药组与西药组间NO含量相比,差异无统计学意义(P>0.05)。结论安宫牛黄丸结合西药应用于原发性高血压脑出血急性期可减轻急性期脑组织损伤,其作用机制可能与减少血NO含量有关。
Objective To explore the intervention of angong niuhuang wan on tissue cell apoptosis,the expressions of NF-κB and the content of NO around hematoma after spontaneous hypertensive cerebral hemorrhage.Methods The injection of autologous blood was adopted to prepare the model of cerebral hemorrhage.Model group,Chinese medicine group,western medicine group and integrated Chinese and western medicine group were divided for the research of intervention treatment.IHC method was used to determine the expressions of NF-κB and the content of NO in serum.Results The expression of NF-κB in integrated Chinese and western medicine group,Chinese medicine group and western medicine group was higher apparently as compared with model group（P〈0.05）,and there was no statistical difference among treatment groups（P〉0.05）.The serum NO content in integrated Chinese and western medicine group was lower apparently as compared with other three groups（P〈0.05）.Serum NO content of brain tissue in Chinese medicine group,western medicine group and integrated Chinese and western medicine group was lower than that in model group（P〈0.05）.There was no statistical significant difference in NO content in comparison between Chinese medicine group and western medicine group（P〉0.05）.Conclusion Angong Niuhuang Wan combined with western medicine can alleviate the acute brain tissue injury in spontaneous hypertensive cerebral hemorrhage of acute stage and its mechanism is probably relevant with the decrease of serum NO content.
出处
《世界中西医结合杂志》
2011年第1期19-21,共3页
World Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金项目(No.30760315)
