酶解法从猪皮中提取胶原的工艺优选

Optimization of Extraction Process of Collagen from Pigskin by Enzyme Hydrolysis

目的:筛选蛋白酶酶解猪皮制备胶原的最佳工艺;方法:以胶原得率为指标,采用单因素试验及正交试验对酶解条件进行优选;结果:胃蛋白酶用量为3%,酶解液pH 2.0,底物质量浓度1:28,温度0℃,酶解时间48 h时胶原的得率最高;结论:胃蛋白酶酶解猪皮制备胶原的工艺较好。

Objective：Screen the optimum preparation for collagen by enzyme hydrolysis.Method： Enzy-matic condition was optimized by single-factor experiments and the orthogonal design with collagen yield as the in-dex.Result： The optimum extraction condition was obtained as follows：the ratio of enzyme /substrate was 3%,pH 2.0,material-liquid ratio 1∶ 28,enzymatic hydrolyzed for 48 h at 0 ℃.Conclusion：Preparation for pigskin collagen by pepsin is better.