柿叶多糖对糖尿病小鼠降血糖作用及其机制研究

Hypoglycemic Effect of Persimmon Leaf Polysaccharide in Diabetic Mice Induced by Streptozotocin

目的:研究柿叶多糖对链脲佐菌素糖尿病小鼠的降血糖作用及其作用机制。方法:将实验小鼠随机分成5组:正常对照组、模型组、格列本脲组(25 mg.kg-1.d-1),柿叶多糖低、高剂量组(150,600 mg.kg-1.d-1)。ig给于药物15 d,并在相应时间采血测定空腹血糖(FBG)、空腹胰岛素(FINS)、胰岛素敏感指数(ISI)、丙二醛(MDA)、超氧化歧化酶(SOD)等指标。结果:高剂量的柿叶多糖能较明显的改善糖尿病小鼠的葡萄糖耐量(P<0.01),低、高剂量组均能显著降低糖尿病小鼠的血糖水平(P<0.05),有效降低肝组织MDA值,相应增高SOD活力,并能显著增加胰岛素敏感指数(ISI),改善胰岛素抵抗状态。结论:柿叶多糖具有较强的降血糖作用,可能与其能提高小鼠肝脏抗氧化酶活性、改善胰岛素抵抗等有关。

Objective：To investigate the hypoglycemic effect and its action mechanism of persimmon leaf polysaccharide in diabetic mice induced by streptozotocin（STZ）.Method：The diabetic mice were induced by STZ.Mice were randomly divided into 5 groups：control group,model group,glibenclamide group（25 mg·kg-1·d-1）,low and high dose groups of persimmon leaf polysaccharide（150 mg·kg-1·d-1,600 mg·kg-1·d-1）.The drugswere administrated to mice in every morning for 15 days.In the corresponding time,the contents of fasting blood glu-cose（FBG）,fasting insulin（FINS）,malondialdehyde（MDA）,superoide dismutase（SOD）activity,insulin sensi-tivity index（ISI）and oral glucose tolerance test（OGTT）were determined.Result：The persimmon leaf polysaccha-ride could markedly decrease blood glucose in STZ-induced diabetic mice.Meanwhile it could reduce the content ofMDA and increase the activity of SOD in liver,and increase insulin sensitivity index（ISI）and the amount of isletcells.Conclusion：The persimmon leaf polysaccharide decreases blood glucose,and it has a very potential value inthe treatment of diabetes.