肾形肾状线虫的生活史及繁殖能力

Life cycle and reproductive capacity of Rotylenchulus reniformis

为明确肾形肾状线虫(Rotylenchulus reniformis)的生活史及在植物根部的繁殖情况,以2龄幼虫接种大豆幼苗,定期取样,通过次氯酸钠-酸性品红染色方法系统观察肾形肾状线虫在寄主根内的侵染及发育,同时,以10、1001、000和10 000条·盆-12龄幼虫接种大豆幼苗,80 d后统计土壤中线虫及根系统中雌虫、卵囊和单卵囊内卵的个数,数据采用SAS 9.0软件分析.结果表明:在(27±2)℃条件下,肾形肾状线虫整个生活史约为27 d;80 d后,不同接种量的土壤线虫量、单卵囊内卵的个数,线虫总量及繁殖系数均存在明显差异;根系统中雌虫和卵囊数量、土壤中线虫数量及线虫总量随着接种线虫量的增加而增加,但单卵囊内卵的个数和繁殖系数却随接种量的增加呈降低趋势,其中繁殖系数最大值为140.4,最小值为2.9,最大值约为最小值的48倍;大豆生长受到影响,植株鲜质量和干质量随接种量的增加而减少.

To find out life cycle and reproductivity of Rotylenchulus reniformis,soybean seedlings were inoculated with second stage juveniles （J2） of R.reniformis,and the parasitic situation and development of R.reniformis on the soybean roots were observed systematically by the periodical sampling and using NaClO-acid fuchsin-glycerin staining technique.At the same time,the reproduction of R.reniformis on the soybean including the number of the nematodes in soil,females and egg masses of root system,eggs per egg mass,and fresh mass and dry mass of soybean shoots and roots were measured at 80 days after inoculation with 10,100,1 000 and 10 000 J2·pot-1,and all statistical analysis were conducted with SAS version 9.0.The results indicated that life cycle of R.reniformis was about 27 d at（27±2） ℃ for soybean.The numbers of the nematodes in soil,eggs per egg mass,final nematode numbers and reproductive factors were significantly different after 80 days of inoculation with different inoculum levels.The reproductive factors,eggs per egg mass decreased with an increase in the inoculum levels,whereas the others were increased with increase of the inoculum levels.The highest reproductive factor was about 48 times of the lowest one,with the highest and the lowest being 140.4 and 2.9,respectively.The growth of soybean was affected,and its fresh and dry mass was decreased with increase of the inoculum levels.