高效液相色谱法同时测定血浆中组织胺和去甲肾上腺素

Simultaneous Determination of Histamine and Noradrenaline in Human Plasma by High performance Liquid Chromatography

建立了用高效液相色谱—电化学检测器（（ＨＰＬＣ／ＥＣＤ）、柱前衍生法，同时测定生物样本中组织胺（Ｈｉｓｔ）和去甲肾上腺素（ＮＡ）的方法．可在２０ｍｉｎ内分离检测出Ｈｉｓｔ和ＮＡ，线性范围为０．０１５～５０ｍｇ／Ｌ，最低检出限为１５ｎｇ／Ｌ，回收率为９５％，精密度为５．８％～１１．１％．具有灵敏、稳定、特异性高等特点．应用检测了７０例健康人、５例高血压病患者血浆中Ｈｉｓｔ和ＮＡ的含量。

A method of simultaneous determination of Histamine (Hist) and Noradrenaline (NA) in human plasma was established to study the decomposing relationship of Hist and NA. The assay was based on precolumn derivatization of Hist and NA ,internal standard with 3 methylhistamine (3 Me hist), separation of the derivatives by high performance liquid chromatographic method with electrochemical detection (HPLC/ECD ).This complete separation of 3 kinds of component was achieved within 20 min .The assay was linear from 0.015～50ng/ml.The minimal detection limit was 15ng/L.The recovery of 3 kinds of component added to plasma samples was 95%.The precision was 5.8%～11.1%. HPLC/ECD method has many advantages in rapidity, simplicity, accuracy, sensitivity and higher distinction. The experimental results have indicated that the distribution of Hist parallels with that of NA in human plasma.