导入高梁DNA选育丰产、抗逆小麦新品系及其RAPD分子验证

Selection and RAPD Identification of a New Wheat Line with High Yield and Stress Resistance by Introducing Sorghum DNA

通过花粉管通道法，将高粱ＤＮＡ导入小麦，结果在其后代产生了广泛的变异，并从中选育出高产、抗逆、耐盐碱小麦新品系８９１２２．在省级区试中，连续两年表现优良，比统一对照高原６０２增产２４．０８％，比原受体陇春１３号增产２１．０６％．在盐碱地，比当地主栽品种Ｖ２８增产１０．５％，比原受体增产２２．５％．光合效率明显提高．ＲＡＰＤ分析结果表明，新品系基因组出现多态性，并具有供体特异性ＤＮＡ带。

The DNA extracted from the sorghum seeding was introduced into the embryos of wheat through a pollentube channel after self-pollination.A great range of variations appeared in the progeny of the recipient,from which a new wheat line 89122 with high yield and stress resistance was selected.The fine traits of this new line are as follows:(1)In Gansu test fields, its yield was the highest among 12 fine accessions or lines in last two years.The yield was 24.08% higher than that of CK plateau 602,and 21.08% higher than that of recipient Longchun 13.(2)In saline soil test,the yield was 10.5 % higher than that of local salt-resistance variety V 28 , and 22.5 % higher than that of the recipient.(3)Photosynthetic efficiency increased significantly. Random amplified polymophic DNA(RAPD) analysis revealed that the new line DNA polymorphism displayed in 3 primers from 60 primers tested.Therefore, the new line with a series of fine traits and specific characters of sorghum was resulted from the sorghum gene introduced.