亳菊香料超声波提取工艺优化及挥发性成分分析

Optimization of Ultrasonic Extraction Conditions of Chrysanthemum morifolium Ramat Flavor Extract and the Analysis of Volatile Composition

以亳菊香料浸膏提取率为考察指标,采用L9(34)正交实验法进行优选。采用同时蒸馏萃取装置收集挥发性成分并用气相色谱-质谱仪进行分析和鉴定。最佳提取条件为:超声波功率800 W,提取时间30 min,乙醇体积分数70%。共鉴定出88种化合物,用峰面积归一化法测定各组分的峰面积百分比为:顺-香橙烯9.38%、n-棕榈酸5.11%、β-人参烯4.63%、长叶醛4.36%、α-雪松烯氧化物3.97%、桉双烯酮3.67%、异香橙烯环氧化物3.35%、1-氟-2-(Z-2-甲氧基)苯3.17%、α-衣兰烯3.15%、亚油酸2.68%、异香橙烯2.54%、α-香附酮2.35%等。优选得到的工艺可提高亳菊香料浸膏的提取率。

Taking the extraction efficiency of Chrysanthemum morifolium Ramat flavor extract as the index,the L9（34） orthogonal experiment was applied to optimization.The volatile compositions in the perfume were prepared with the simultaneous distillation and extraction equipment（SDE）.They were isolated and indentified by the capillary GC-MS method.The optimal extraction conditions were obtained as followed：φ（alcohol）=70%,ultrasonic power 800 W and time 30 min.88 peak compounds were isolated and identified.The relative contents of the total peak area were determined by the area normalization method.The main volatile compositions were：（＋）-aromadendrene（9.38%）,n-hexadecanoic acid（5.11%）,β-panasinsene（4.63%）,longifolenaldehyde（4.36%）,α-cedrene oxide（3.97%）,eudesma-4（14）,11-diene（3.67%）,isoaromadendrene epoxide（3.35%）,1-fluoro-2-（Z-2-methoxyethenyl）benzene（3.17%）,α-ylangene（3.15%）,linolic acid（2.68%）,alloaromadendren（2.54%）,α-cyperone（2.35%）,etc.The optimum process increased the extraction efficiency of Chrysanthemum morifolium Ramat flavor extract.