丁酸钠对宫颈癌HeLa细胞增殖及血管内皮生长因子表达的影响

Effect of sodium butyrate on proliferation of cervical cancer HeLa cell line and VEGF gene expression

目的观察丁酸钠对宫颈癌HeLa细胞的数量及血管内皮生长因子（VEGF）mRNA表达水平的影响，探讨丁酸钠对HeLa细胞增殖的作用及相关机制。方法体外分组培养的HeLa细胞分别加入不同浓度的丁酸钠作用72h后，用四甲基偶氮唑盐比色法（MTT）检测细胞生长情况，逆转录聚合酶链反应（RT—PCR）检测各组细胞VEGFmRNA表达情况。结果经不同浓度的丁酸钠作用后，宫颈癌HeLa细胞增殖水平及VEGFmRNA表达水平较对照组均有明显降低，在一定范围内呈现浓度依赖性。结论丁酸钠不但能够抑制宫颈癌HeLa细胞的增殖，还可抑制宫颈癌HeLa细胞VEGF基因的表达水平。丁酸钠抑制VEGF基因表达水平的作用可能是其抑癌机制之一。

Objective To detect the effect of sodium butyrate on proliferation of cervical cancer HeLa cell line and VEGF mRNA expression and investigate the role of sodium butyrate in the proliferation of the cervical cancer HeLa cell line. Methods Cervical cancer HeLa cell line was cultured in vitro. After treatment with various concentrations of sodium butyrate for 72 h, MTT was used to detect the proliferative and inhibitive status. And VEGF mRNA - expression was detected by RT - PCR. Results After treatment with varions concentrations of sodium butyrate, the cell proliferation and VEGF mRNA expression was obviously depressed, compared with the control group, the effect in a time - dose - dependent manner within a certain range. Conclusions Sodium butyrate can inhibit cell proliferation and VEGF gene expression level in cervical cancer HeLa cell line. Inhibition of the VEGF gene expression level may be one of the reasons that sodium butyrate inhibits the proliferation of the cervical cancer HeLa cell line.