基于血红蛋白/纳米金/二氧化锆-壳聚糖修饰的过氧化氢生物传感器

A Biosensor for Hydrogen Peroxide Based on Hemoglobin Peroxidase Immobilized on Nano-Au/ Chitosan-ZrO_2/gold Electrode

将二氧化锆(ZrO2)分散于壳聚糖(CS)中得到稳定的CS-ZrO2复合物,制备了Hb/nano-Au/CS-ZrO2/Au过氧化氢生物传感器。用循环伏安法和计时电流法考察该修饰电极的电化学特性,发现该修饰电极对过氧化氢(H2O2)的还原有良好的电催化作用。实验结果表明,该传感器对H2O2的线性响应范围为.9×10-6～1.8×10-3 mol/L,线性相关系数R=0.9956,检测下限为5.6×10-7 mol/L(S/N=3),并具有选择性高、线性范围宽和响应快等优点。

A new approach to measure with hemoglobin （Hb）/nano-Au/chitosan （CS）-ZrOJAu modified electrode was established. Zirconium dioxide（ZrO2） was dispersed in the chitosan（CS） to form a stable CSZrO2 composite. This composite was first coated on the surface of gold electrode to provide an amino group interface for assembling nano-Au, followed by the adsorption of Hb to form a bilayer of Hb/nano-Au. The Hb/nano-Au/CS-ZrO2/gold electrode was used as hydrogen peroxide （HEO2 ） sensor. Cycling voltammetric and chronoamperometric characterizations reveal that the modified electrode exhibit electrocatalytic behavior to H2O2. The results confirmed that the electrode exhibited high sensitivity, fast response, and linear response in the range of 3.9× 10^-6 -1.8 × 10^-3 mol/L（R =0. 9956） with the lowest detection limit of 5.6 × 10^-7 mol/L （S/N = 3）.