HPLC法同时测定旋覆花配方颗粒中绿原酸及咖啡酸含量

Determination of chlorogenic acid and caffeic acid in Xuanfuhua dispensing granule by HPLC

目的：建立同时测定旋覆花配方颗粒中绿原酸及咖啡酸含量的HPLC方法。方法：采用正交试验优化旋覆花配方颗粒的提取工艺,用HPLC法测定旋覆花配方颗粒中绿原酸及咖啡酸的含量。色谱柱为Shim-packVP-ODS柱（250 nm×4.6 mm,5μm）;流动相为乙腈-0.1%磷酸（9∶91）;检测波长为327 nm;流速为1.0 mL.min-1。结果：绿原酸在0.86-2.58μg（r=0.999 9）范围内线性关系良好,平均回收率为102.95%,RSD为1.21%;咖啡酸在0.28-1.10μg（r=0.999 9）范围内线性关系良好,平均回收率为99.72%,RSD为1.64%。结论：该方法简便、快速、重复性好,可作为旋覆花配方颗粒中绿原酸及咖啡酸的含量测定方法。

Objective：To develop a HPLC method for the simultaneous determination of chlorogenic acid and caffeic acid in Xuanfuhua dispensing granule.Methods：The orthogonal test was used to optimize extract conditions.Chlorogenic acid and caffeic acid were separated on Shim-pack VP-ODS（250 mm×4.6 mm,5 μm） column and detected at 327 nm,with Acetonitrile-0.1% phosphoric acid solution（9∶91） as mobile phase.The flow rate was 1.0 mL·min-1.Results：The method had good linear relationship in the range of 0.86~2.58 μg（r=0.999 9） for chlorogenic acid and 0.28~1.10 μg（r=0.999 9） for caffeic acid,respectively.The results of the average recovery were 102.95% with RSD of 1.21% for chlorogenic acid and 99.72% with RSD of 1.64% for caffeic acid.Conclusion：The method was simple and reliable which can be used to simultaneously determine chlorogenic acid and caffeic acid in Xuanfuhua dispensing granule.