实时荧光定量PCR法在检测泌尿生殖道感染病原体中的应用

Application of fluorescent quantitative PCR to detect pathogens in urogenital tract

目的评价荧光定量PCR(FQ-PCR)在实验室检测泌尿生殖道病原体、淋病奈瑟菌(NG)、解脲脲原体(UU)和沙眼衣原体(CT)中的应用。方法 464例拟诊为泌尿生殖道感染者的临床标本,用培养法检测NG、UU病原体和胶体金免疫层析法检测CT抗原,对同一标本同时用FQ-PCR检测NG、UU或CT的核酸。结果 FQ-PCR和培养法检出NG阳性率分别为28.4%(63/222)和14.9%(33/222),P<0.01,检测UU的阳性率分别为41.7%(126/302)和32.5%(98/302),P<0.05,FQ-PCR和金标法检测CT阳性率分别为18.2%(57/314)和6.1%(19/314),P<0.001,FQ-PCR对3种病原体的检出率均明显高于培养法或金标法。结论 FQ-PCR法检测NG、CT以及UU在临床诊断NG,UU和CT感染中具有重要应用价值,是常规方法的有效补充。

Objective To assess the application of fluorescent quantitative PCR（FQ-PCR） to detect Neisseria gonorrhoeae （NG）, Ureaplasma urealyticum （UU） and Chlaydia trachomatis （CT） in urogenital tract. Methods Culture method for detection of NG and UU pathogens, GICA for CT antigens were applied in 464 clinical samples from patients with suspected urogenital tract infection. At the same time, all DNA of NG, UU or CT from the same samples were amplified by FQ-PCR. Results The FQ-PCR and culture method showed positive rates of 28.4%（63/222）and 14.9%（33/222）,P0.01 in NG detection, 41.7%（126/302）and 32.5%（98/302）,P0.05 in UU detection, respectively. FQ-PCR and GICA indicated positive rates of 18.2%（57/314） and 6.1%（19/314）,P0.001 in CT detection. FQ-PCR assays revealed much higher sensitivity than culture method or GICA in this study. Conclusion It is suggested that FQ-PCR shows important practical value in diagnosis of NG, UU and CT infection, as an effective complement to routine methods.