丹酚酸A抗过氧化鼠肝损伤的作用

Antiperoxidation effects of salvianolic acid A on CCl4 induced liver injury

目的观察丹酚酸Ａ（ＳＡＡ）抗过氧化肝损伤的作用．方法采用ＣＣｌ４诱导大鼠肝损伤模型，观察ＳＡＡ对肝脏病理、血清Ａｌｂ含量与ＡＬＴ，ＡＳＴ活性，肝组织ＭＤＡ含量的影响．另以ＣＣｌ４熏蒸诱导肝细胞损伤，观察ＳＡＡ对ＡＬＴ，ＡＳＴ，ＧＳＨＰＸ的活性以及对ＭＤＡ，ＧＳＨ含量的变化．结果ＳＡＡ能减轻模型鼠肝脏病理变化，降低ＡＬＴ，ＡＳＴ活性与ＭＤＡ含量；降低损伤肝细胞ＡＬＴ，ＡＳＴ，ＳＯＤ，ＧＳＨＰＸ活性与ＭＤＡ含量，增高ＧＳＨ含量．结论ＳＡＡ具有良好的抗过氧化肝损伤作用．

AIM To investigate the antiperoxidation effects of salvianolic acid A(SAA) on liver injury. METHODS Liver injury models were induced by carbon tetrachloride (CCl4) in rats in vivo, and treated with Salvianolic acid A. Histopathologic changes of the liver samples, serum albumin contents, serum alanine aminotransferase (ALT) and asparate aminotransferase (AST) activities, malondialdehyde (MDA) contents in the livers were observed. The primarily cultured hepatocytes were injured by CCl4 gas in vitro, and incubated with salvianolic acid A. Influence of the drug on the activities of ALT, AST and GSHPX, and on contents of MDA and glutathione (GSH) were observed. RESULTS SAA could alleviate the histopathological changes, decreased ALT and AST activities and MDA content in the liver injured rats in vivo. It also could decrease ALT, AST, SOD and GSHPX activities, decrease MDA contents, and improve GSH contents in CCl4 gas injured cultured hepatocytes in vitro. CONCLUSION SAA has a good action against peroxidation in the liver injury.