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临床常见念珠菌的PCR-RFLP鉴定方法研究 被引量：1

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摘要 目的建立临床常见念珠菌的聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测技术。方法用真菌通用引物ITS1-ITS4分别扩增白色念珠菌、热带念珠菌、光滑念珠菌、克柔念珠菌和近平滑念珠菌5种菌株的ITS1-5.8SrDNA-ITS2区域,然后对扩增产物进行MspⅠ酶切分析。结果 5种念珠菌标准菌株经PCR-RFLP分析后产生了5种不同的特异性条带,成功地将临床上常见的5种念珠菌区分开来。应用此方法鉴定了60株临床分离的念珠菌,其PCR-RFLP结果与标准菌株一致,测序结果进一步证实了此方法的可靠性。结论 PCR-RFLP检测技术具有快速、稳定、特异、准确性高的特点,在常见致病念珠菌鉴定方面具有良好的临床应用前景。
作者 窦娟 张全斌 金柳 张景萍 王萍 商润萍 郑新萍 周永安
机构地区 山西医科大学 太原市中心医院检验科
出处 《中华临床医师杂志（电子版）》 CAS 2011年第1期263-266,共4页 Chinese Journal of Clinicians(Electronic Edition)
关键词 念珠菌属 聚合酶链反应
分类号 R440 [医药卫生—诊断学]
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参考文献11

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共引文献11

同被引文献15

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中华临床医师杂志（电子版）
2011年 第1期

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