短链脂肪酸对TPN大鼠小肠粘膜结构及细胞增殖作用的研究

Influence of short chain fatty acids(SCFAs) on small intestine morphology and epithelial cells proliferation in TPNfed rats

目的：探讨短链脂肪酸（ＳＣＦＡｓ）对ＴＰＮ大鼠小肠粘膜的保护作用。方法：将３０只近交系Ｗｉｓｔｅｒ（ＲＴ１Ｋ）大鼠随机分为假手术对照组、常规ＴＰＮ组及ＳＣＦＡｓ－ＴＰＮ组，进行为期１４天实验，结束后观察小肠粘膜的形态学变化和细胞增殖情况。结果：常规ＴＰＮ组大鼠肠粘膜绒毛高度、宽度及隐窝深度较正常对照组均有显著减少，其增殖指数也显著降低；ＳＣＦＡｓ－ＴＰＮ组上述指标明显升高，与对照组基本接近。结论：短链脂肪酸（ＳＣＦＡｓ）能促进ＴＰＮ大鼠小肠粘膜细胞增殖，维持其正常形态，从而有保护肠粘膜机械屏障的作用。

Objectives: Total pareteral nutrition (TPN) has proved to be a valuable method of nutrtion support.However,when supplid as the sole source of nutrition to normal animals ,TPN produced atrophy of the small intestine. SCFAs were shown to stimulate mucosal cell mitotic activity,this study determined whether the addition of SCFAs could prevent intestine mucosal atrophy produced by TPN. Methods: Thirty adult Wistar(RT1K) rats were clarified into three groups.With ten rats served as sham operation control .Tewty rats had catheters inserted into the superior vena cava,of which ten was sustained with classical TPN and others with TPN plus SCFAs(sodium acetate,propionate,and butyrate) for 14 days. Smll intestine villi hight,width,crypt depth,and epithelial cell proliferation index(PI) were study and compared. Results: Animals receiving only classical TPN had significant decreas of small intestine villi hight(162.818.3) m, width(71.414.5) m and crypt depth(73.28.2) m. Epithelial cell proliferation index(PI) was also very low(3.761.42) compared to control(5.512.14).In SCFAsTPN treated rats, morphological parameters and proliferation index were all much higher than classical TPNfed rats.There were no apparent difference between SCFAsTPN group and sham operation control group. Conclusions: The results suggest that SCFAs may be used to decrease the mucosal atrophy that commonly occurs after TPN support and other gastrointestinal inhibition state.