摘要
重组葡激酶突变体基因在大肠杆菌中高效表达，产物以包涵体的形式存在于胞浆中。通过发酵，扩增工程菌，机械破菌，离心分离粗制包涵体，后者经纯化后用盐酸胍溶解，ｒ－ＳＡＫΔＮ分子复性后用离子交换层析和分子筛脱盐，纯度达９７％，比活性５×１０４ＨＵ／ｍｇ，Ｍｒ１４０００，等电点为ｐＨ６．８。
Recombinant staphylokinase mutant (rSAKN) was efficiently expressed in E coli in the form of
inclusion body By the methods of fermentation, inclusion body extraction, refolding of rSAKN
molecular, Qsepharose FF ionexchange chromatography and gel filtration of Sephadex G25 The
purity of rSAKN was reached 97% and with specific activity 5104 HU/mg The Mr and isoelectro
point were 14000 and pH68 respectively
出处
《药物生物技术》
CAS
CSCD
1999年第2期65-69,共5页
Pharmaceutical Biotechnology
关键词
葡激酶
突变体
包涵体
纯化
rStaphylokinase mutant (rSAKN), Inclusion
body, Purification
