摘要
在对ＤＭＥＭ：Ｆ１２（１∶１）进行优化的基础上，以细胞生长和组织型纤溶酶原激活剂（ｔ－ＰＡ）表达为评价指标，筛选无血清培基添加成分，建立了由优化ＤＭＥＭ：Ｆ１２（１：１）、胰岛素、ＰｌｕｒｏｎｉｃＦ－６８、维生素Ｃ、乙醇胺和微量元素混合液组成的适于产ｔ－ＰＡＣＨＯ工程细胞４Ｂ３生长和ｔ－ＰＡ表达效果均优于相同条件下用含血清培基培养的效果。
Based on the optimization of DMEMF12(11), and the selection of supplements for serumfree medium using the cell growth and the tPA expression as the evaluation indexes, a serumfree medium, DF5S, which consisted of optimized DMEMF12(11), insulin, pluronic F68, vitamin C, trace element mix and ethanolamine, for the growth of geneticallyengineered CHO cell line 4B3 was formulated Immobilized cultivation of 4B3 cells with Cytopore in spinner flasks, the growth and tPA production of 4B3 in DF5S were superior to that observed in serumcontaining medium
出处
《药物生物技术》
CAS
CSCD
1999年第2期85-89,共5页
Pharmaceutical Biotechnology
基金
"863"中试研究项目
