极大螺旋藻胞外多糖EPⅡ的分离、纯化及免疫学研究

Isolation,Purification and lmmunological Activities of Extracellular Polysaccharide EP from Spirulina Maxima

极大螺旋藻培养液经浓缩，脱盐，醇沉得胞外粗多糖（ＥＰＩ）。ＥＰⅠ依次经ＤＥ－５２和ＳｅｐｈａｄｅｘＧ－１００纯化得纯品多糖（ＥＰⅡ）。经ＳｅｐｈａｄｅｘＧ－２００检测，ＥＰⅡ为均一组分，Ｍｒ６００００。薄层层析、气相层析、红外光谱分析表明，ＥＰⅡ是由Ｌ－岩藻糖，Ｄ－甘露糖，Ｄ－半乳糖，Ｄ－葡萄糖和葡萄糖醛酸组成的酸性杂多糖，糖苷键为β型。免疫调节活性试验表明，ＥＰⅡ有提高小鼠的ＮＫ细胞杀伤活力，促进ＩＬ－２产生及淋巴细胞和混合淋巴细胞转化功能。

The raw extracellular polysaccharide(EP)was isolated from the fermentative solution through concentratingdesalting and precipitating with ethanal separately.EP was further purified by loading on DE52 and Sephadx G100 column chromatography,so EP was obtained.EP was identified by sephadex G200 to be homogenous,with the molecular weight 6.0104.The ultraviolet spectrum of EP indicated that nucleic acid and protein are absent.The data of IR revealed that EP is chiefly linked by glycosidic bonds.Chemical composition analysis by TLC and GC showed that EP is composed of Lfucose,Dmannose,Dgalatose,Dglucose and glucoronic acid.Immunological activity assays indicated that EP could improve IL2 production,killing activity of NK cells,lymphocyte transformation and mixed lymphocyte response transformation in mice.