胆道梗阻后肝细胞线粒体钙含量变化和肝脂质过氧化损害的实验研究

EXPERIMENTAL STUDY ON THE CHANGE OF HEPATOCYTE MITOCHONDRIAL CALCIUM CONTENT AND LIVER LIPIDPEROXIDATION AFTER BILIARY OBSTRUCTION

为了解胆道梗阻对肝脏的损害机理，在复制大鼠胆道梗阻模型基础上，分离肝细胞线粒体，动态检测肝细胞线粒体钙含量，肝组织ＭＤＡ、ＳＯＤ含量，血清ＴＢｉｌ、ＡＬＴ、ＡＬＰ及ＧＧＴ含量。结果：肝细胞线粒体钙含量、肝组织ＭＤＡ含量和血清ＴＢｉｌ、ＡＬＴ、ＡＬＰ及ＧＧＴ水平均随梗阻时间延长而逐渐升高（Ｐ＜０．０５），肝组织ＳＯＤ含量则逐渐减少（Ｐ＜０．０５）；肝细胞线粒体钙含量与肝组织ＭＤＡ含量、血清ＡＬＴ及ＡＬＰ含量变化呈明显正相关，ｒ分别为０．９６７、０．９２４和０．９１９（Ｐ＜０．０１）；肝组织ＭＤＡ含量与血清ＡＬＴ和ＡＬＰ含量变化呈明显正相关，ｒ分别为０．９４９和０．８４３（Ｐ＜０．０１）。结论：肝细胞线粒体钙超载和脂质过氧化损伤密切相关，在胆道梗阻所致肝损害过程中起重要作用。

To investigate the mechanisms of hepatic injury after biliary obstruction. After a rat model of complete biliary obstruction(CBO) was induced, hepatocyte mitochondria was isolated and the calcium content of mitochondria, the contents of liver malondialdyhyde (MDA) and superoxide dismutase (SOD), the levels of serum TBil, ALT, ALP and GGT were measured in each group. Results: After CBO, mitochondrial calcium content, liver MDA and serum TBil, ALT, ALP, GGT became increased progressively, compared with control group (P<0.05); the liver SOD was decreased markedly (P<0.05). Mitochondrial calcium content was highly positively correlated with liver MDA content, serum ALT and ALP, r values were 0.967, 0.924 and 0.919 respectively (P<0.01). The liver MDA content was highly positively correlated with serum ALT and ALP, r values were 0.949 and 0.843 respectively (P<0.01). Conclusions: Mitochondrial calcium overload and liver lipid peroxidation may be the important mechanisms of hepatic injury induced by biliary obstruction.