丹参多酚酸B对动脉粥样硬化大鼠炎症细胞因子的影响

Effects of Salvianolic Acid B on Inflammatory Cytokines in Atherosclerosis Rats

目的:研究丹参多酚酸B对动脉粥样硬化大鼠炎症反应相关细胞因子的影响。方法:8周龄雄性SD大鼠60只,随机分成6组(正常组,模型组,丹参多酚酸B低剂量组、中剂量组、高剂量组,辛伐他汀组),并给予相应的处理。电镜下观察心肌超微结构,并取心肌组织用酶联免疫吸附法(ELISA)检测肿瘤坏死因子α(TNF-α)、白细胞介素1α(IL-1α)、白细胞介素6(IL-6)相关炎性因子。结果:各用药组线粒体肿胀以及线粒体嵴排列紊乱等情况较模型组都有明显好转。模型组心肌TNF-α、IL-1α、IL-6浓度均明显高于正常组(P<0.05);丹参多酚酸B不同浓度组以及辛伐他汀组心肌TNF-α浓度显著低于模型组(P<0.05);丹参多酚酸B不同浓度组以及辛伐他汀组心肌IL-1α浓度均有下降,但其差异无统计学意义(P>0.05);丹参多酚酸B中剂量和高剂量组以及辛伐他汀组心肌IL-6浓度显著低于模型组(P<0.05)。结论:丹参多酚酸B具有降低TNF-α、IL-6分泌的作用,从而抑制动脉粥样硬化的炎症反应。

Objective： To observe the effects of salvianolic acid B（Sal B） on the inflammatory cytokines in atherosclerosis rats.Methods： Sixty male SD rats were randomly divided into 6 groups（with 10 rats in each group）： normal group,model group,low-dosage Sal B group,middle-dosage Sal B group,high-dosage Sal B group and simvastatin group;each group was treated with corresponding diets and/or medicine.The myocardiac ultrastructure was observed by electron microscopy and the contents of such inflammatory cytokines as TNF-α,IL-1α and IL-6 were detected by enzyme-linked immunosorbent assay（ELISA）.Results： Compared with model group,the swelling of myocardial mitochondrion and poor alignment of mitochondrial crista were relieved in the other groups.The contents of myocardial TNF-α,IL-1α and IL-6 were higher in the model group than in the normal group（P0.05）;the content of TNF-α was lower in the Sal B groups and simvastatin group than in the model group（P0.05）;the content of IL-1α was insignificantly lowered in the Sal B groups and simvastatin group（P0.05）;the content of myocardial IL-6 was significantly lower in the middle-and high-dosage Sal B groups and simvastatin group than in the model group（P0.05）.Conclusion： Salvianolic acid B can reduce the secretion of TNF-α and IL-6 to inhibit the inflammatory reaction in atherosclerosis.