木蹄层孔菌石油醚组分的成分分析及抗肿瘤活性研究

Analysis of chemical constituents of petroleum ether fraction from the fruiting body of Fomes fomentarius and its antitumor effect in vitro

目的:分析木蹄层孔菌(Fomes fomentarius)石油醚组分中甾醇和三萜类等成分及其主要抗肿瘤活性物质。方法:采用95%乙醇回流提取法及拌硅胶淋洗方法得到木蹄层孔菌的石油醚组分。用气相色谱-质谱联用技术对石油醚组分甾体和三萜类成分进行分析和鉴定并计算出其含量。用硅胶柱色谱法分离纯化含量最高的7,22-二烯麦角甾-3-酮。采用Alamar Blue法进行体外抗肿瘤活性检测。结果:从木蹄层孔菌石油醚组分中检测出37个成分,鉴定了其中的35个成分,包括10种甾体和三萜类成分。其主要成分为:7,22-二烯麦角甾-3-酮(37.67%)和5,24(28)-二烯麦角甾-3-醇(27.01%)。石油醚组分对细胞株NCI-H460的IC50为75μg.mL-1,7,22-二烯麦角甾-3-酮对细胞株NCI-H460的IC50为30μg.mL-1,抗肿瘤活性与单体浓度存在明显的量效关系。结论:木蹄层孔菌石油醚组分主要成分为7,22-二烯麦角甾-3-酮和5,24(28)-二烯麦角甾-3-醇,抗肿瘤活性物质最高的为7,22-二烯麦角甾-3-酮。研究结果为木蹄层孔菌的进一步开发提供了依据。

Objective：To analyze chemical constituents of petroleum ether extract from the fruiting body of Fomes fomentarius and ascertain the main antitumor constituent.Methods：The fruiting body of Fomes fomentarius was extracted by alcohol reflux.The extracts was absorbed by silica gel,then eluted by petroleum ether,ethyl acetate and methanol respectively.Silica gel column was resorted to isolate and purify the compound ergosta-7,22-dien-3-one the highest constituent of the petroleum ether extract.The antitumor activity was detected by Alamar Blue method in vitro.Results：37 compounds from the petroleum ether extract were separated and identified by GC-MS.35 of them were determined,10 of which were determined as sterols and triterpene compounds.And their relative contents were also assayed.The main constituents and higher relative content have been identified as ergosta-7,22-dien-3-one（37.67%）,ergosta-5,24（28）-dien-3-ol（27.01%）.The antitumor activity of petroleum ether extract was strong against NCI-H 460 cell line,the inhibiting rate was 89.04% at 100 μg·mL-1,and IC50 value of petroleum ether extract was 75 μg·mL-1.The IC50 value of ergosta-7,22-dien-3-one was 30 μg·mL-1.Conclusions：The main compounds of petroleum ether extract from the fruiting body of Fomes fomentarius were ergosta-7,22-dien-3-one and ergosta-5,24（28）-dien-3-ol.And ergosta-7,22-dien-3-one had strong antitumor activity against NCI-H 460 cell line.