一种改进的大脑皮层神经元原代培养方法的研究

Research on an improved method for primary culture of cerebral cortex neurons

目的:建立1种简单、高效的新生乳鼠大脑皮层神经元原代培养的方法。方法:出生12 h以内的SD乳鼠为研究对象,采用胰酶消化和机械分离法相结合制备细胞悬液;在细胞培养过程中,不加入阿糖胞苷抑制神经胶质细胞生长,而是用2%B27 Neurobasal A medium维持培养。结果:神经元生长良好,形成神经元网络系统;通过神经元免疫组织化学鉴定神经元纯度达90%以上。结论:此方法是获得纯度较高的神经元的1种可靠方法。

Objective：To establish an simple and effective primary culture skill suitable to neurons of newborn rat cortical tissure in vitro.Method：Objects of study were newborn SD rats which born within 12 h,combination trypsin digestion and mechanical dissociation were adopted to conduct culture;in the course of culture,arabinosid cytarabine（Arc-C） was not put to use to inhibit the outgrowth of glia cell,2%B27 Neurobasal A medium maintain cell growth.Result：The neurons grew well and formed nerver net system;purity of cultured neurons exceeds 85% by immunohistochemical identification.Conclusion：The technique is a reliable way to obtain higher purity neurons.