摘要
目的:建立1种简单、高效的新生乳鼠大脑皮层神经元原代培养的方法。方法:出生12 h以内的SD乳鼠为研究对象,采用胰酶消化和机械分离法相结合制备细胞悬液;在细胞培养过程中,不加入阿糖胞苷抑制神经胶质细胞生长,而是用2%B27 Neurobasal A medium维持培养。结果:神经元生长良好,形成神经元网络系统;通过神经元免疫组织化学鉴定神经元纯度达90%以上。结论:此方法是获得纯度较高的神经元的1种可靠方法。
Objective:To establish an simple and effective primary culture skill suitable to neurons of newborn rat cortical tissure in vitro.Method:Objects of study were newborn SD rats which born within 12 h,combination trypsin digestion and mechanical dissociation were adopted to conduct culture;in the course of culture,arabinosid cytarabine(Arc-C) was not put to use to inhibit the outgrowth of glia cell,2%B27 Neurobasal A medium maintain cell growth.Result:The neurons grew well and formed nerver net system;purity of cultured neurons exceeds 85% by immunohistochemical identification.Conclusion:The technique is a reliable way to obtain higher purity neurons.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第2期299-301,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金(30770579)
973项目(2007C13936101)
863项目(2007AA021901)
中国药品生物制品检定所中青年研究发展基金(2009C4)资助
关键词
神经元
细胞培养
乳鼠
neuron
cell culture
newborn rat