HPLC切换波长法同时测定升麻中5种成分的含量

HPLC wavelength switching simultaneous determination of the content for 5 kinds of constituents in cimicifuga

目的:建立以切换波长的方法同时测定升麻中咖啡酸、升麻素苷、阿魏酸、异阿魏酸、升麻素5种成分含量的方法。方法:采用反相高效液相色谱法,Agilent Eclipse XDB-C18(4.6 mm×150 mm,5μm)色谱柱;流动相:乙腈-0.1%磷酸水溶液(13:87);检测波长:0～7 min,323 nm;7～12 min,254 nm;12～17 min,316 nm;17～20 min,254 nm;柱温:40℃;流速:1.0 mL.min-1;进样量:10μL。结果:不同商品地的升麻中5种成分的含量差异显著,升麻素苷和升麻素在部分样品中未检出。结论:切换波长法可用于升麻中5种成分含量的同时测定,为升麻质量评价提供了科学依据。

Objective：To establish an RP-HPLC method of wavelength switching simultaneous determination of the content for caffeic acid,cimicifuga glycodides,ferulate acid,iso-ferulic acid,Cimicifuga Su 5 kinds of constituents in cimicifuga.Methods：Agilent Eclipse XDB-C18（4.6 mm×150 mm,5 μm）columns;mobile phase：acetonitrile-0.1% phosphoric acid solution（13∶87）;the detection wavelength：0-7 min,323 nm;7-12 min,254 nm;12-17 min,316 nm;17-20 min,254 nm;column temperature：40 ℃;velocity：1.0 mL·min-1;the injection volume：10 μL.Results：Prim places in different commodities 5 kinds of constituents in significantly different cimicifuga glycodides and Cimicifuga Su were undetected in some samples.Conclusion：The method of wavelength switching can be used for the simultaneous determination 5 kinds of constituents in cimicifuga,which provide a scientific basis for quality assessment to Cimicifuga.