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产甲酸草酸杆菌FRC基因慢病毒表达载体构建及鉴定 被引量:1

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摘要 产甲酸草酸杆菌(Oxalobacter{ormigenes,m.F)是一种寄居于脊椎动物胃肠道、依赖分解肠道草酸生存的革兰阴性菌。我们采用基因克隆重组技术从前期分离培养的中国人肠道Ox.F[3-中克隆了草酸分解关键酶——甲酰辅酶A转移酶(FCoAT)基因FRC,并成功构建重组慢病毒表达载体pLenti6.3FRC—IRES-EGFP,为高草酸尿症基因治疗提供基础。
出处 《中华泌尿外科杂志》 CAS CSCD 北大核心 2011年第2期141-142,共2页 Chinese Journal of Urology
基金 基金项目:国家自然科学基金(NO.30872564)
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