微波辅助碱液提取仙草多糖

Microwave-assisted Alkali Extraction of Mesona Blumes Polysaccharides

研究微波辅助碱液提取仙草多糖的工艺条件。考虑沸水浴时间、碱液浓度、料液比和微波处理时间4个因素对仙草粗多糖提取率的影响,通过单因素试验选择各因素较理想的4个水平,再通过正交试验优化工艺参数。结果表明:碱液浓度是提高仙草多糖提取率的最显著因素,其次是料液比,微波处理时间和沸水浴时间对提取率的影响相对较小。在微波功率400W条件下,碱液浓度0.125mol/L、微波时间90s、料液比1:35(mg/mL)、水浸提1h时仙草粗多糖提取率最高,达到43.84%。

The microwave-assisted alkali extraction of polysaccharides from Mesona Blumes was optimized in this paper. To do this,the effects of four factors including water-bath boiling time,alkaline concentration,material/liquid ratio and microwave treatment time on the extraction rate of polysaccharides were explored by single-factor and orthogonal design methods. Results indicated that alkaline concentration was the most critical factor affecting the extraction rate of polysaccharides,followed by material/liquid ratio. Microwave treatment time and water-bath boiling time exhibited less effect on the extraction rate of polysaccharides. The optimal extraction conditions were microwave treatment time of 90 s,extraction power of 400 W,water-bath boiling time of 1 h,alkaline concentration of 0.125 mol/L,and material/liquid ratio of 1：35. Under these optimal conditions,the extraction rate of polysaccharides from Mesona Blumes was up to 43.84%.