响应面试验优化苹果渣微晶纤维素制备工艺

Optimization of the Preparation of Microcrystalline Cellulose from Apple Pomace

研究利用苹果渣纤维素制备微晶纤维素的方法过程。在水解时间50min、水解温度100℃条件下,采用响应曲面设计法设计、分析,研究酸水解纤维素制备微晶纤维素时,各因素料液比、盐酸质量分数和次氯酸钠添加量及3因素间两两交互作用对微晶纤维素得率的影响。结果表明,各因素对得率影响的显著性表现为盐酸质量分数>料液比>次氯酸钠添加量。分析等高线和响应面,得出各因素两两之间的交互作用对得率都显著。通过求解回归方程得出最佳工艺条件为料液比1:25(g/mL)、盐酸质量分数6%、次氯酸钠添加量2.5mL/100mL,此条件下微晶纤维素制备得率达到69.5%。

Rinsing with sodium hypochlorite and hydrolysis with hydrochloric acid are two key procedures for the preparation of microcrystalline cellulose from apple pomace. In the present study, based on single factor experiments, by which hydrolysis time of 50 min and temperature of 100℃ were determined, the pairwise interactive effects of material/liquid ratio, hydrochloric acid concentration and the amount of sodium hypochlorite addition on microcrystalline cellulose yield were discussed by central composite design （CCD） in combination with response surface analysis. The significance of the above three factors affecting microcrystalline cellulose yield decreased in the following order： hydrochloric acid concentration 〉 material/liquid ratio 〉 the amount of added sodium hypochlorite. Analysis based on response surface and contour plots showed that all the pairwise interactions significantly affected microcrystalline cellulose yield. The regression equation based on the results from CCD design was resolved, and the results indicated that the optimal amount of added sodium hypochlorite for rinsing apple cellulose was 2.5 mL/100 mL, and that the optimal hydrolysis effect was achieved by the use of 6% hydrochloric acid to hydrolyze the substrate diluted 25-fold by volume. Under these conditions, a rnicrocrystalline cellulose yield of 69.5% was obtained.