薤白抗菌物质的提取工艺

Process Optimization for Aqueous Extraction of Anti-Staphyloccocus aureus Substances from Allium macrostemon Bunge

为优化薤白中抗菌物质的提取工艺条件,采用单因素试验和响应面分析方法,以水为提取剂,以金黄色葡萄球菌抑菌圈直径为响应值,确定最佳的薤白抗菌物质的水浸提条件:加水量为薤白浆液体积的2倍,32℃提取4次,每次3h。此条件下得到的抗菌物质抗菌性较好,进行金黄色葡萄球菌的抑菌实验,得到抑菌圈直径达到13.98mm。证明薤白具有抑菌活性,所提取的抗菌物质可以作为天然食品防腐剂。

Previous studies have showed that Allium macrostemon Bunge is a broad-spectrum antimicrobial plant. In the present study,crude aqueous extracts isolated from Allium macrostemon Bunge under different levels of water amount as well as extraction time,temperature and number were tested comparatively for their anti-Escherichia coli and anti-Staphyloccocus aureus activities,and the plant showed better inhibitory effect against Staphyloccocus aureus than against anti-Escherichia coli. To obtain highly active anti-Staphyloccocus aureus substances from Allium macrostemon Bunge,the above four extraction conditions were optimized by response surface methodology based on inhibition circle diameter against Staphyloccocus aureus. The optimum levels of water amount as well as extraction time,temperature and number were determined to be 2-fold volume,3 h （each time）,32 ℃ and 4,respectively,and under these conditions an extract with an inhibition circle diameter of 13.98 mm was obtained. Therefore,Allium macrostemon Bunge has the potential to be developed into a natural food preservative.