响应面分析法优化超声波提取槟榔原花青素工艺

Process Optimization for Ultrasonic-assisted Solvent Extraction of Proanthocyanidins from Areca catechu L. Fruit by Response Surface Analysis

利用响应面分析法(RSM)对槟榔原花青素的提取工艺进行优化。在单因素试验基础上选取试验因素与水平,根据中心组合(Box-Behnken)试验设计原理采用四因素三水平的响应面分析法对各个因素的显著性和交互作用进行分析,结果表明:槟榔干原花青素提取的最佳工艺条件为超声功率600W、超声时间16min、料液比1:40(g/mL)、丙酮体积分数81%,在此条件下,测定花青素提取率最高达到3.41%。

The purpose of the current study was the optimization of the process for the solvent extraction of proanthocyanidins from Areca catechu L. fruit with ultrasonic assistance. First of all,the selection of extraction solvent was investigated. Secondly,one-factor-at-a-time （OFAT） experiments were done to figure out the effects of five process parameters for the extraction of proanthocyanidins with acetone aqueous solution,including acetone concentration in aqueous solution,extraction temperature,material-to-liquid ratio,ultrasonic power and ultrasonic treatment time on the extraction rate of proanthocyanidins. Thirdly,the relationship of the extraction rate of proanthocyanidins with the other four extraction conditions except extraction temperature was mathematically modeled on the basis of a four-variable,three-level central composite design （CCD） with 26 runs. Finally,the model established was subjected to analysis of variance to test the significance of various variables and response surface analysis to understand their pairwise interactive effects on the function. From the above investigations and analysis,we determined the optimum conditions for the extraction of proanthocyanidins from Areca catechu L. fruit to be：ultrasonic power of 600 W,ultrasonic treatment time of 16 min,the ratio of material to solvent of 1：40 （g/mL） and acetone concentration of 81%. Under these conditions,the maximum extraction rate of proanthocyanidins was up to 3.41%.