响应面法对大豆胰蛋白酶抑制剂粗提工艺的优化

Process Optimization for Trypsin Inhibitor Extraction from Defatted Soybean Meal by Response Surface Methodology

目的:对大豆胰蛋白酶抑制剂粗提工艺进行优化。方法:以提取液pH值、热变性温度和硫酸氨饱和度作为影响大豆胰蛋白酶抑制剂提取率的因素,通过单因素试验选取因素与水平,根据Box-Behnken中心组合试验设计原理,在单因素试验基础上采用三因素三水平响应面分析法,依据回归分析确定各工艺条件的主要影响因素,以大豆胰蛋白酶抑制剂提取率为响应值作响应面分析。结果:胰蛋白酶抑制剂提取的最佳工艺条件为提取液pH5.1、热变性温度72.8℃、硫酸氨饱和度54.5%,在此条件下,胰蛋白酶抑制剂的提取率达到86%。结论:采用响应面分析法可以对大豆胰蛋白酶抑制剂粗提工艺进行优化。

Objective：The conditions for the extraction of trypsin inhibitor from low-temperature defatted soybean meal were optimized. Methods：pH,thermal denaturation temperature and ammonium sulfate saturation were identified to be main variables that influence extraction efficiency by the one-factor-at-a-time method. Based on the principles of Box-Behnken central composite design,response surface analysis was employed to explore the effects of pairwise interactions among the three variables at three levels on extraction efficiency. The optimum conditions for extracting soybean trypsin inhibitor from low-temperature defatted soybean meal were found as follows：extraction solvent pH of 5.1,thermal denaturation at 72.8 ℃ and ammonium sulfate saturation of 54.5% for the precipitation of trypsin inhibitor. Under these conditions,the extraction efficiency of trypsin inhibitor was up to 86%. Consequently,response surface methodology is feasible for the optimization of the extraction process for trypsin inhibitor from defatted soybean meal,and the optimized process is reliable.