不同蚕蛹油中脂肪酸的性状及组分分析

Character and Fatty Acid Composition of Silkworm Pupal Oil

研究不同蚕蛹油的脂肪酸种类和相对含量。采用气相色谱-质谱方法,对KOH/CH3OH酯交换法甲酯化4种不同的蚕蛹油进行定量分析。结果表明:柞蚕蛹、蓖麻蚕蛹、家蚕蛹及剿丝后家蚕蛹油的酸价、皂化值和过氧化值存在显著差别;4种蛹油的脂肪酸成分的种类都以棕榈酸、硬脂酸、油酸、亚油酸、α-亚麻酸为主,但含量存在显著区别;4种蚕蛹油中,柞蚕蛹油的不饱和脂肪酸相对含量是最高的,达77.29%;蓖麻蚕蛹油的α-亚麻酸相对含量最高,为50.52%;缫丝后家蚕蛹油不饱和脂肪酸的相对含量比缫丝前降低。

In order to determine the types and relative contents of fatty acids in 4 kinds of silkworm pupal oil,oil transesterification was carried out with KOH/CH3OH,followed by gas chromatography-mass spectrometric analysis. Results indicated that acid values,saponification values and peroxidation values differed significantly among the 4 kinds of pupal oil. All the four tested materials contained the following major components：linolenic acid （LNA）,palmitic acid,stearic acid,oleic acid andα-linolenic acid,which exhibited different relative contents. Among them,the relative content of unsaturated fatty acids in oak silkworm pupal oil was the highest,which was up to 77.29% and castor silkworm pupal oil contained the mostα-linolenic acid,with a relative content of 50.52%. Desilked pupal oil contained more unsaturated fatty acids than silkworm pupal oil.