昆明系小鼠生物法定量测定水产品中河豚毒素

Quantitative Detection of Tetrodotoxin Using Kunming Strain Mice

为建立昆明系小鼠定量测定河豚毒素(TTX)方法,选用体质量19～21g的昆明系小鼠作为实验对象,用不同质量浓度TTX溶液腹腔注射小鼠。结果显示:TTX致昆明系小鼠的半数致死量(LD50)为0.18μg,小鼠死亡时间与TTX注射剂量满足方程Y=e(0.357/X+1.005)。此外,采用体积分数0.5%乙酸溶液提取TTX,回收率达到90%以上,对样品的最低检测限为0.56μg/g。昆明系小鼠定量测定河豚毒素,准确、稳定、方便快捷,可用于水产品安全检测。

This study was aimed at establishing a laboratory method for the quantitative detection of tetrodotoxin （TTX）. A bioassay was developed by injecting intraperitoneally TTX at various dosages to Kunming strain male mice with body weights of 19-21 g. Results indicated that the 50% lethal dose （LD50） of TTX for Kunming strain mice was 0.18μg. The relationship between TTX dosage and the mean death time of Kunming mice could be expressed as Y = e（0.357/ X ＋1.005）. In addition,a high recovery rate of TTX was achieved by using 0.5% （V/V） acetic acid to extract TTX from globefish,which reached up to 90%. However,the detection limit of the assay for samples was 0.56μg/g. Therefore,Kunming strain mice can be used to quantitatively determine TTX in an accurate,stable and convenient manner,which is promising in the safety control of fishery products.