摘要
为建立昆明系小鼠定量测定河豚毒素(TTX)方法,选用体质量19~21g的昆明系小鼠作为实验对象,用不同质量浓度TTX溶液腹腔注射小鼠。结果显示:TTX致昆明系小鼠的半数致死量(LD50)为0.18μg,小鼠死亡时间与TTX注射剂量满足方程Y=e(0.357/X+1.005)。此外,采用体积分数0.5%乙酸溶液提取TTX,回收率达到90%以上,对样品的最低检测限为0.56μg/g。昆明系小鼠定量测定河豚毒素,准确、稳定、方便快捷,可用于水产品安全检测。
This study was aimed at establishing a laboratory method for the quantitative detection of tetrodotoxin (TTX). A bioassay was developed by injecting intraperitoneally TTX at various dosages to Kunming strain male mice with body weights of 19-21 g. Results indicated that the 50% lethal dose (LD50) of TTX for Kunming strain mice was 0.18μg. The relationship between TTX dosage and the mean death time of Kunming mice could be expressed as Y = e(0.357/ X +1.005). In addition,a high recovery rate of TTX was achieved by using 0.5% (V/V) acetic acid to extract TTX from globefish,which reached up to 90%. However,the detection limit of the assay for samples was 0.56μg/g. Therefore,Kunming strain mice can be used to quantitatively determine TTX in an accurate,stable and convenient manner,which is promising in the safety control of fishery products.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第4期181-184,共4页
Food Science
基金
2009年国家质量监督检验检疫总局出入境检验检疫行业标准制(修)订计划项目(2009B138)