摘要
采用聚酰胺吸附法提取样品溶液的色素,用乙醇-氨水-水混合溶液解吸,用高效液相色谱-二极管阵列检测器测定。采用ODS柱分离,以甲醇和硫酸铵溶液为流动相进行洗脱,在520nm波长处检测。亮黑、荧光素钠、红色2G、荧光桃红和孟加拉玫瑰红的质量浓度在1.0～10.0mg/L范围内与其色谱峰面积的线性关系良好(r=0.999),在1、2、5mg/kg添加水平时的平均回收率在82.95%～95.43%范围内,RSD值在1.99%～5.95%范围内。方法检出限为1.0mg/kg。该方法准确度高,分离效能好,结果稳定可靠,适合于饮料和糖果中亮黑、荧光素钠、红色2G、荧光桃红和孟加拉玫瑰红的测定。
A quantitative determination method for Brilliant Black PN,Fluorescein Sodium,Red 2G,Phloxine B and Rose Bengal in drink and candy was established using high performance liquid chromatography （HPLC）. For sample preparation,extraction by polyamide adsorption and desorption with ethanol-ammonia solution-water （7：2：1,V/V） were carried out. An ODS C18 column （250 mm × 4.6 mm,5μm） was used for chromatographic separation,which was eluted with methanol-0.02 mol/L ammonium acetate aqueous solution as mobile phase at 35 ℃. The linear range of the developed method was 1.0-10.0 mg/L with relative standard deviation of 1.99%-5.95% and the detection limit was 1.0 mg/kg. The average recovery rates were 82.95%-95.43%. This method is simple,accurate,and suitable for the determination of these illegally-added colorants in drink and candy.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第4期189-192,共4页
Food Science
基金
中国检验检疫科学院基本科研业务费专项(2009JK011)
