限制性液体复苏对失血性休克大鼠肝脏一氧化氮合酶表达的影响

Effects of restricted fluid resuscitation on expression of liver nitric oxide synthase in rats with hemorrhagic shock

目的探讨限制性液体复苏对失血性休克大鼠肝脏一氧化氮合酶表达的影响。方法60只sD大鼠制成未控制性重度失血性休克模型，随机分成对照组、常规组（常规大量液体复苏组）和限制组（限制性液体复苏组），检测和比较休克复苏后各组存活大鼠血清NO的含量和肝脏组织eNOS蛋白、iNOS蛋白和eNOSmRNA、iNOSmRNA的表达。结果失血性休克大鼠限制组血清NO含量和肝脏组织eNOS蛋白和eNOSmRNA的表达较常规组显著升高；与对照组比较，常规组血清NO含量和肝脏组织eNOS蛋白和eNOSmRNA的表达升高；常规组肝脏组织iNOS蛋白和iNOStuRNA的表达较对照组低，与常规组、对照组比较，限制组肝脏组织iNOS蛋白和iNOSmRNA的表达显著降低，以上差异均有统计学意义（P均〈0．05）：结论限制性液体复苏可以显著改善失血性休克大鼠肝脏损伤中微循环的紊乱，其机制可能与增强肝脏组织中eNOS表达、提高血清NO含量有关。

Objective To investigate the effects of restricted fluid resuscitation on expression of liver nitric oxide synthase in rats with hemorrhagic shock. Methods A model of rat with severe hemorrhagic shock caused by active bleeding was established in 60 SD rats. Those rats were randomly divided into control group, restricted group （restricted fluid resuscitation group） and conventional group （conventional large - volume fluid resuscitation group）. After different treatments, survival rats in each group were detected and compared of NO in serum, the expressions of NOS mRNA and protein respectively. Results NO in serum, the mRNA and protein expressions of eNOS in liver of restricted group were obviously higher than those of conventional group（ P 〈 0.05）. Compared with control group, NO in serum, the mRNA and protein expressions of eNOS in liver of conventional group were significantly increased （P 〈 0.05）. The mRNA and protein expressions of iNOS in liver of conventional group were significant- ly lower than those of control group （ P 〈 0.05 ）. Compared with conventional group and control group, the mRNA and protein expressions of iNOS in liver of restricted group were obviously decreased （P 〈 0.05 ）. Conclusions Restricted fluid resuscitation can significantly protect liver from microcirculation disorder injury. The mechanism might be related with the induction of eNOS in tissue and the increase of NO in