食管鳞癌张力蛋白同源物磷酸脂酶基因蛋白及基质金属蛋白酶-7的表达和临床意义

Expression and significance of PTEN and MMP -7 in esophageal squamous cell carcinoma

目的探讨张力蛋白同源物磷酸脂酶基因（PTEN）蛋白及基质金属蛋白酶-7（MMP-7）的表达与食管癌发生、发展及浸润、转移的关系。方法应用免疫组织化学法检测61例食管鳞癌组织、30例癌旁不典型增生组织及60例正常食管黏膜组织中PTEN蛋白及MMP-7蛋白的表达。结果食管鳞癌组织中PTEN蛋白与MMP-7蛋白的表达与癌的组织学分级、浸润深度及淋巴结转移密切相关（P〈0．01）；在食管上皮癌变过程中PTEN蛋白在正常黏膜组织、癌旁不典型增生组织及癌组织中的表达率依次降低，分别为93．3％（56／60）、73．3％（22／30）、54．1％（33／61），纽间比较差异有统计学意义（P〈0．01）；而MMP-7蛋白在癌组织及癌旁不典型增生组织表达率显著高于正常黏膜组织，分别为42．6％（26／61）、33．3％（10／30）、18．3％（11／60），组间比较差异有统计学意义（P〈0．01）；PTEN蛋白与MMP-7的表达呈负相关（P〈0．01）。结论PTEN蛋白和MMP-7在食管癌的浸润、转移及黏膜上皮癌变过程中起重要作用，PTEN蛋白及MMP-7的联合检测有望成为食管鳞癌早期诊断和判断预后的分子指标之一。

Objective To investigate the significance and expression of phosphatase and tensin homology deleted on chromosome ten （PTEN） and matrix metalloproteinase - 7 （ MMP - 7） in esophageal squamous cell carcinoma. Methods S -P immunohistochemistry was employed to detect the expression of PTEN and MMP -7 in 61 cases of esophageal squamous cell carcinoma,30 cases of periearcinoma e- sophageal tissue and 60 cases of normal esophageal mucosa. Results The expression of PTEN and MMP- 7 in esophageal squamous cell carcinoma was closely correlated with tumor histological grade, depth of invasion and lymph node metastasis （ P 〈 0.01 ）. The expression rates of FFEN in normal esophageal epithelium specimens, adjacent atypical hyperplasia epithelium and carcinoma reduced in turn which were 93.3% （56/60） ,73.3% （22/30） ,54.1% （33/61） respectively. And there were significant differences in different groups （ P 〈 0.01 ）. The positive rate of MMP -7 protein expression in cancer tis- sue and mucosa adjacent to cancer were higher than that in normal mucosa tissue which were 42.6% （26/61）, 33.3 % （ 10/30 ）, 18.3 % （11/60） respectively. And there were significant differences in different groups （P 〈 0. 01 ）. There was negative correlation between the expression of PTEN and MMP- 7. Conclusions FTEN and MMP- 7 play important roles in infiltration, metastasis and mucous membrane epidermis canceration process of esophageal molecular indexs in the early diagnosis and prognostic carcinoma. PTEN and MMP -7 can become one of judgement of esophageal squamous cell carcinoma.