长段神经液氮冷冻预防残端神经瘤形成的实验研究

Experiment studies on the preventing the forming of terminal neuromas by cryotherapy of a long segment nerve with liquid nitrogen in rats

目的 探讨长段神经液氮冷冻预防残端神经瘤形成的效果。方法 实验用 3 0只SD大鼠 ,左侧坐骨神经切断并切除残端 6mm、神经残端液氮冷冻 12± 1mm ,右侧同样切断但不冷冻设为对照侧 ,观察 2 8周 ,采用肉眼及光镜组织学的方法进行评价。结果 3 0例对照侧均有坐骨神经瘤形成 ,而神经残端长段经冷冻处理后未见神经瘤形成。

Objective To explore the effect of preventing the forming of terminal neuromas by cryotherapy of a long segment nerve with liquid nitrogen. Methods Thirty Sprague-Dawley rats were used in this study. The left lateral sciatic nerves were transected and a length of 6 mm of the terminal part were removed, then a length of 12±1 mm of the terminal part were cryolesioned by liquid nitrogen. As a control group, the right lateral sciatic nerves were transected together. Histological study and naked eye surveying were performed after 28 weeks. Results The sciatic neuromas were showed in the control group, on the contrary, no typical neuromas were showed in the left lateral sciatic nerves.Conclusion The method of a long segment nerve cryotherapy by liquid nitrogen can prevent the forming of neuroma.