米非司酮对早孕绒毛及蜕膜细胞周期动力学的影响

Effect of Mifepristone on Cellcycle Kinetics of Human Placental Choriotrophoblastic and Decidual Cell

目的探讨米非司酮对早孕绒毛及蜕膜细胞周期动力学的影响。方法应用流式细胞技术对２３例正常早孕和２４例口服米非司酮后吸宫流产物的绒毛及蜕膜组织，进行细胞周期动力学分析。结果服用米非司酮后，绒毛滋养层细胞Ｇ０、Ｇ１期细胞明显增加（Ｐ＜０．０１），Ｇ２、Ｍ期明显降低（Ｐ＜０．０１），细胞增殖指数明显降低（Ｐ＜０．０１）；蜕膜细胞除Ｇ２、Ｍ期细胞上升（Ｐ＜０．０５）外，其余各期无变化。结论米非司酮可阻断滋养层Ｇ０、Ｇ１期细胞向Ｓ期的转化，从而抑制细胞增殖，且其对绒毛的作用大于对蜕膜的作用。

Objective To study the effects of mifepristone on cellcycle kinetics of human placental choriotrophoblastic and decidual cells. Methods Flowcytometry was introduced in the study of the mechanism of early pregnancy terminating drug, mifepristone.Twentythree samples were obtained from normal pregnant women by surgical aspiration. Another 24 samples were obtained in women pretreated with 150 mg mifepristone 48 hours before operation. Results The percentage of the cytotrophoblastic cells in G0 +G1 phases was much higher in the treatment group (P<0.01), whereas it was much lower (P<0.01) in G2 +M phases, and the proliferating index of cells was signifreantly decreased. Apart from the increasing of decidual cells in G2 +M phases(P<0.05), there was no change in other phases. 5HZConclusions Mifepristone may block the transformation of the cytotrophoblastic cells from G0, G1 phases to S phase,thus leading to the block of the proliferation of cells. Mifepristone has much more significant effects on the chorionic villi than on the decidua.