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非类固醇类抗炎药NS398对肺癌H460细胞MT1-MMP表达的影响 被引量：2

Effect of NS398 on the expression of membrane type 1-matrix metalloproteinase in human H460 lung carcinoma cells
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摘要 目的:探讨非类固醇类抗炎药NS398对肺癌H460细胞增殖及其膜型基质金属蛋白酶-1(mem-brane type 1-matrix metalloproteinase,MT1-MMP)表达的影响。方法:应用MTT法检测细胞生长抑制率,免疫荧光法检测MT1-MMP蛋白质表达,酶联免疫吸附法(enzyme-linked immunosorbentassay,ELISA)检测细胞培养液中活性基质金属蛋白酶-2(matrixmetalloproteinase-2,MMP-2)的浓度。结果:NS398可抑制H460细胞的增殖及MT1-MMP蛋白质的表达,减少H460细胞培养液中活性型MMP-2的含量,并呈剂量依赖关系。结论:NS398可能通过抑制肺癌细胞MT1-MMP表达及MMP-2的激活而抑制肺癌细胞的侵袭转移。 Objective： To explore the effect of NS398 on the expression of membrane type 1 - matrix metalloproteinase （MT1 -MMP） and the activation of matrix metalloproteinase- 2 （MMP- 2） in human H460 lung carcinoma cells. Methods： The H460 cell growth inhibition rate was detected by MTT assay, Immunofluorescence assay was used to analyse the expression of MT1 - MMP protein levels in H460 cells. Enzyme - linked immunosorbentassay was used to detect the actions of NS398 on the activation of MMP -2 in H460 cells. Results： Treatment with NS398 in H460 cells caused a dose - dependent decrease in the expression of MT1 - MMP protein. NS398 also caused a decrease in the expression of MMP -2 protein in H460 cells. Conclusion： NS398 might decrease the expression of MT1 - MMP and MMP - 2 activation in lung carcinoma cells, which can lead to the decrease of extracellularmatrix followed by acceleration of tumor cell migration and invasion. It is probably an key mechanism of NS398 regulated lung carcinoma cell migration.
作者 范红燕 朱运奎 李继东 杨娜 张冬泉
机构地区 兰州大学第二临床医学院 兰州军区兰州总医院呼吸内科
出处 《现代肿瘤医学》 CAS 2011年第2期235-238,共4页 Journal of Modern Oncology
基金 甘肃省自然科学基金项目(编号:SZS051-A25-088)
关键词 氮-2 环己氧-4 硝基苯-甲基磺胺 人肺癌细胞H460 膜型基质金属蛋白酶-1 基质金属蛋白 酶-2 NS398 H460 lung carcinoma cells membrane type 1 - matrix metalloproteinase matrix metalloprotease-2
分类号 R730.23 [医药卫生—肿瘤]
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参考文献16

  • 1Elder DJ, Hague A, Hicks DJ, et al. Differential growth inhibition by the aspirinmeta - bolite saliclate in human colorectal tumor cell lines: enhanced apoptosis in carcinoma and in vitro transformed adenoma relative to adenoma cell lines[ J]. Cancer Res, 1996, 56 (10) : 2773 -2776.
  • 2Nelson JE, Harris RE. Inverse association of prostate cancer and nonsteroidal antiinflammatory drugs results of case - control study [ J ]. OncolRep, 2000, 7 ( 1 ) : 169 - 170.
  • 3谢泽明,朱志华,张兰军,戎铁华,吴秋良.Ⅰ期非小细胞肺癌中侵袭与转移相关蛋白的表达及其预后意义[J].第四军医大学学报,2008,29(8):743-745. 被引量：6
  • 4Adolphie M, Deysson G, Lechat P. Action of some steroid and non- steroid anti - inflammatory agents on cell cycle: cytophotometric study of DNA content [ J ]. Rev Eur Rtud Clin Biol, 1972,17:320.
  • 5Fujima T, Matsui M, Takaku K, et al. Size - and invasion - dependent increase in cyclooxygenase 2 levels in human colorectal carcinomas [ J]. Cancer Res, 1998,58:4823.
  • 6Sano H, Kawahito Y, Wilder RL, et al. Expression of cyclooxygenase- 1 and -2 in human colorectal cancer [ J ]. Cancer Res, 1995,55:3785.
  • 7Appleby SB, Ristimaki A, Neilson K, et al. Structure of human cyclo - oxygenase - 2 gene [ J ]. Biochem J, 1994, 302 ( 3 ) : 723 - 727.
  • 8Morita I, Schindler M, Regier MK, et al. Differential intracellular locations for prostaglandin endoperoxideH synthase - 1 and - 2 [J]. J Biol Chem, 1995, 270(18) : 10902 -10908.
  • 9Sternlicht MD, Bergers G. Matrixmetalloproteinases as emerging targets in anticaneer therapy: status and prospects [ J]. Emerg Therapeut Targets, 2000, 4 (5) : 609 -633.
  • 10Mc Cawley LJ, Matrisian LM. Matrixmetalloproleinases : they're not just formatrix anymore[J]. Curr Opin Cell Biol, 2001, 13 (4) : 534 -54.

二级参考文献18

  • 1Kononen J,Bubendorf L,Kallioniemi A,et al.Tissue microarrays for high-throughput molecular profiling of tumor specimens[J].Nat Med,1998,4(7):844-847.
  • 2Kim SJ,Rabbani Z,Dewhirst MW,et al.Expression of HIF-1a,CAIX,VEGF,and MMP-9 in surgically resected non-small cell lung cancer[J].Lung Cancer,2005,49:325-335.
  • 3Passlick B,Sienel W,Seen-Hibler R,et al.Overexpression of matrix metalloproteinase 2 predicts unfavorable outcome in early-stage non-small cell lung cancer[J].Clin Cancer Ros,2000,6:3944-3948.
  • 4Ishikawa S,Takenaka K,Yanagihara K,et al.Matrix metalloprotoinase-2 status in stromal fibroblasts,not in tumor cells,is a significant prognostic factor in non-small-cell lung cancer[J].Clin Cancer Res,2004,10:6579-6585.
  • 5Sienel W,Hellers J,Morresi-Hauf A,et al.Prognostic impact of matrix metalloproteinase-9 in operable non-small cell lung cancer[J].Int J Cancer,2003,103:647-651.
  • 6Ramasami S,Kerr KM,Chapman AD,et al.Expression of CD44v6 but not Ecadherin or b-catenin influences prognosis in primary pulmomary adenocarcinoma[J].J Pathol,2000,192:427-432.
  • 7Pirinen RT,Hirvikoski P,Johansson RT,et al.Reduced expression of acatenin,b-catenin,and c-catonin is associated with high cell proliferative activity and poor differentiation in non-small cell lung cancer[J].J Clin Pathol,2001,54:391-395.
  • 8Kase S,Sugio K,Yamazaki K,et al.Expression of E-cadherin and betacatenin in human non-small cell lung cancer and the clinical significance[J].Clin Cancer Res,2000,6:4789-4796.
  • 9Lee YC,Wu CT,Chen CS,et al.The significance of E-cadherin and a-,b-,and c-catenin expression in surgically treated non-small cell lung cancers of 3 cm or less in size[J].J Thorac Cardiovaec Surg,2002,123:502-507.
  • 10Bremnes RM,Veve R,Gabrielson E,et al.High-throughput tissue microarray analysis used to evaluate biology and prognostic significance of the E-cadherin pathway in non-sinail-cell lung cancer[J].J Clin Oncol,2002,20:2417-2428.

共引文献5

同被引文献32

  • 1殷积彬,刘铁夫,刘伟,赵瑞波.NS-398影响基质金属蛋白酶-2及其抑制剂的表达[J].中华消化杂志,2006,26(4):262-263. 被引量：3
  • 2冯智英,莫祥兰,邵春奎,苏组兰.黏膜NK/T和成熟T细胞淋巴瘤MMP9表达及其与EBV感染的关系[J].南方医科大学学报,2007,27(9):1338-1340. 被引量：1
  • 3王功成,浦金贤,丁翔.选择性COX-2抑制剂NS398对前列腺癌PC-3细胞增殖与凋亡的影响[J].现代泌尿外科杂志,2007,12(5):292-294. 被引量：4
  • 4Lim WT, Chuah KL, Leong SS, et al. Assessment of human papillo- mavirus and epstein -barr virus in lung adenocarcinoma [ J ]. On- col Rep ,2009 ,21 (4) :971.
  • 5Sela BA. Matrix metalloproteinases: promoters of tumor progression and invasiveness [J]. Isr Med Assoc J, 2002, 4(4): 280-282.
  • 6Groblewska M, Siewko M, Mroczko B, et al. The role of matrix metal- loproteinases (MMPs) and their inhibitors (TIMPs) in the develop- ment of esophageal cancer [J]. Folia Histochem Cytobiol, 2012, 50 (1):12-19.
  • 7Gialeli C, Theocharis AD, Karamanos NK. Roles of matrix metallo- proteinases in cancer progression and their pharmacological targeting [J]. FEBS J, 2011,278(1):16-27.
  • 8Nelson JE, Harris RE. Inverse association of prostate cancer and non-steroidal anti-inflammatory drugs (NSAIDs): results of a case- control study [J]. Oncol Rep, 2000, 7(1): 169-170.
  • 9Ding H, Han C, Zhu J, et al. Celecoxib derivatives induce apoptosis via the disruption of mitochondrial membrane potential and activation of caspase 9[J]. Int J Cancer, 2005, 113(5): 803-810.
  • 10Kessenbrock K, Plaks V, Werb Z. Matrix metalloproteinases: regula- tors of the tumor microenvironment [J]. Cell, 2010, 141(1): 52-67.

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现代肿瘤医学
2011年 第2期

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