槲皮素抑制鱼藤酮诱导的PC12细胞凋亡

Inhibitory effect of quercetin on apoptosis of PC12 cells induced by rotenone

目的:探讨槲皮素对鱼藤酮诱导的PC12细胞凋亡的影响及其作用机制。方法:运用鱼藤酮诱导损伤PC12细胞,经300μmol/L槲皮素预处理后,观察细胞形态学改变,流式细胞术检测细胞凋亡率,Western blot-ting检测Bax和Bcl-2蛋白的表达,JC-1染色检测细胞线粒体膜电位,比较各组的差异。结果:与鱼藤酮组相比,槲皮素加鱼藤酮处理组细胞形态明显改善,凋亡率降至6.3%(P<0.01),Bcl-2表达增加(P<0.01),Bax表达降低(P<0.01),线粒体膜电位上升(P<0.01)。结论:槲皮素对鱼藤酮诱导的PC12细胞凋亡具有抑制作用,上调Bcl-2和下调Bax蛋白的表达,维持线粒体膜电位可能是其作用的机制之一。

AIM：To observe the effects and mechanisms of quercetin on the apoptosis of PC12 cells induced by rotenone.METHODS：PC12 cells were used in the study.Quercetin at the concentration of 300 μmol/L was added into the PC12 cells cultured in DMEM-F12 medium with 10% fetal calf serum.The morphological changes of the cells were observed under fluorescence microscope.The apoptotic rate was determined by flow cytometry assay.The protein levels of Bax and Bcl-2 were determined by Western blotting,and the mitochondrial membrane potential was measured by ratiometric probe JC-1.RESULTS：In the cells treated with rotenone＋quercetin,the morphology of the cells was significantly improved,and the apoptotic rate was decreased to 6.7%,significantly lower than that in the cells treated with rotenone alone（P〈0.01）.The expression of Bcl-2 was up-regulated and Bax was down-regulated in rotenone＋quercetin group（P〈0.01）,while the mitochondrial membrane potential was also increased（P〈0.01） as compared to those in rotenone group.CONCLUSION：Pretreatment of quercetin inhibits the development of apoptosis in PC12 cells induced by rotenone.One of the mechanisms may be correlated with up-regulating the expression of Bcl-2 and down-regulating the expression of Bax,thus maintaining mitochondrial membrane potential.