超排卵对大鼠卵巢组织结构及生殖内分泌的影响

Effects of superovulation on histological changes and functions of reproductive endocrine in rat ovary

目的:探讨超排卵对大鼠卵巢组织结构和生殖内分泌功能的影响,为临床合理使用超排卵提供理论依据。方法:30只雌性SD大鼠随机分为3组:实验A组接受孕马血清和人绒毛膜促性腺激素重复6次超排卵,实验B组给予3次生理盐水注射及孕马血清和人绒毛膜促性腺激素重复3次超排卵,对照组接受6次生理盐水注射。15 d及30 d后分别处死各组一半大鼠,观察卵巢组织形态学变化,采用放射免疫法检测血清雌二醇(E2)、卵泡刺激素(FSH)、黄体生成素(LH)水平,并计算FSH/LH水平,比较各组间的差异。结果:实验A和B组大鼠卵巢始基卵泡和初级卵泡计数明显低于对照组(P<0.05),但各组大鼠体内血清基础激素水平无显著差异(P>0.05)。结论:超排卵会引起大鼠卵巢的卵泡发育受限,且随着超排卵次数的增加而加重,但尚未导致生殖内分泌激素水平的显著改变。

AIM：To study the effects of superovulation on the histological changes and reproductive endocrinic functions of rat ovary.METHODS：Thirty female SD rats were randomly divided into 3 groups：the rats in experimental group A were treated with pregnant mare serum gonadotropin（PMSG） and human chorionic gonadotropin（HCG） for 6 times;the rats in experimental group B were treated with normal saline for 3 times,subsequently stimulated with the same reagents in experimental group A for 3 times;the control rats were treated with normal saline for 6 times.Half of the rats were killed on the 15th day of the experiment,and the others were killed at the end of the experiment.The ovarian morphology and follicle number were observed.The serum estrodiol-2（E2）,follicle stimulating hormone（FSH）,luteinizing hormone（LH） and FSH/LH levels were determined by radioimmunoassay.RESULTS：The number of primordial follicles and primary follicles in the two experimental groups were significantly lower than those in control group（P〈0.05）.However,no significant difference of the serum basal hormone level among the 3 groups was observed（P〉0.05）.CONCLUSION：Superovulation can restrain the development of the follicles in a frequency-dependent manner.