甘肃红砂不同种群遗传多样性的ISSR分析

Analysis of genetic diversity in Reaumuria soongorica populations in Gansu using ISSR markers

本实验采用ISSR分子标记技术,对甘肃地区5个种群共50个单株的红砂遗传多样性水平和遗传结构进行了研究。通过实验,从50个引物中筛选出12个重复性高,条带清晰的引物。12条引物共检测到69个位点,其中多态位点有60个,多态位点比率(P)为86.96%。用MarkerⅢ作为分子量标准,检测到甘肃红砂PCR产物的分子量在500～3 000bp。应用遗传多样性分析软件Popgen 32进行分析计算得出:在物种水平上,Shannon多样性指数(I)为0.542 9,Nei基因多样性指数(H)为0.379 0;基因分化系数Gst为0.096 4,基因流Nm为4.685 1,表明甘肃红砂种群遗传分化大部分存在于种群内。在种群水平上,I为0.489 3,H为0.342 4。P、H及I都表明甘肃红砂种群具有较高的遗传多样性。聚类分析还表明甘肃红砂种群的遗传距离与地理距离之间无显著的相关性;甘肃红砂遗传多样性与其本身特性和所处不同种群有关。

Genetic diversity and genetic structure of 5 Reaumuria soongorica populations from Gansu（total about 50 individuals）,were analyzed by ISSR（inter simple sequence repeat） markers.Twelve highly stable and repeatable ISSR primers（selected from 50 primers tested） detected a total of 69 loci of which 60 were polymorphic（86.96% of the total）.The PCR products ranged from 500 to 3 000 bp.At the species level,the Shannon index（I） was 0.542 9,Nei＇s gene diversity index（H） was 0.379 0,the coefficient of gene differentiation（Gst） was 0.096 4,and the gene flow（Nm） was 4.685 1.The software Popgen 32 analysis of the results showed that there was more genetic differentiation within populations than between populations.At the population level,I and H were 0.489 3 and 0.342 4,respectively.All of these results showed that R.soongorica has a high genetic diversity in Gansu.Cluster analysis showed no direct correlation between genetic distance and geographic distance,suggesting that genetic diversity of R.soongorica in Gansu was possibly related to species traits and communities.