高尔夫果岭草坪草上禾本科根结线虫的鉴定

Identification of Meloidogyne graminis on golf greens

2009年,广东省阳江和珠海的2个高尔夫球场果岭草坪出现大面积的斑块状枯死,调查发现这些果岭草的根部具有根结,同时从土壤当中分离到大量的根结线虫2龄幼虫。运用线虫比较形态学,结合同工酶及分子生物学技术对采自这2个高尔夫球场的根结线虫进行鉴定,确定该根结线虫为禾本科根结线虫(Meloidogyne graminis)。禾本科根结线虫病是美国高尔夫球场的一个重要草坪草病害,在我国则是首次发现。

In 2009,yellow patches emerged on golf greens of two golf courses in Yangjiang and Zhuhai of Guangdong Province.Root knots were observed in turfgrass roots of the golf greens,and numerous juvenile second stage（J2） worms belong to the genus Meloidogyne were isolated from soils around turf grass roots.The root knot nematodes were identified by using morphology,isozyme and mtDNA-PCR-sequence.The results indicated the root knot nematode was M.graminis.M.graminis are known to be an important pathogen of golf course greens turf in USA,however the nematode is found firstly on golf course in China.