环磷酰胺治疗小鼠S180肉瘤的体内^(31)P磁共振波谱研究

In vivo studies of cyclophosphamide chemotherapy on mouse S180 sarcoma by 31Pmagnetic resonance spectroscopy

目的：观察环磷酰胺治疗小鼠Ｓ１８０肉瘤的３１ＰＭＲＳ参数变化，以及这些参数的变化是否早于常规观察的瘤体积的变化。方法：利用表面线圈３１ＰＮＭＲ方法，并研究小鼠皮下接种Ｓ１８０肉瘤的体积。结果：对照组的ＰＣｒ／Ｐｉ和βＮＴＰ／Ｐｉ比值降低，而环磷酰胺给药组升高，且给药后２４、４８、７２ｈ时显著高于对照组（Ｐ＜００５）。对照组的ＰＭＥ／βＮＴＰ比值继续升高，而给药组ＰＭＥ／βＮＴＰ比值在给药后２４ｈ、４８ｈ、７２ｈ时均显著低于对照组（Ｐ＜００５）。因此，对照组和给药组的ＰＣｒ／Ｐｉ、ＰＭＥ／βＮＴＰ、βＮＴＰ／Ｐｉ比值在给药后２４ｈ时基本已具有显著性差别，这３个参数的变化均早于肿瘤体积的变化。结论：小鼠Ｓ１８０肉瘤的３１ＰＭＲＳ不仅可以给出定量的结果。

Objective: To investigate the effect of cyclophosphamide (CTX) on the metabolic profile of mouse S180 sarcoma and whether the changes of 31PMRS parameters is earlier than the changes of tumor volume. Methods: Murine S180 sarcoma (s.c.) was studied by using surfacecoil 31PNMR. Results: Twentyfour hours after cyclophosphamide treatment,PCr/Pi and NTP/Pi ratios of CTX treated groups increased significantly relative to control (P< 005).PME/NTP ratio of CTX treated groups was lower than those of control (P< 005). The tumor volume between treated groups and control group showed no significant difference before 72h aflter treatment. Conclusions:31PMRS of mouse S180 sarcoma could not only provide quantitative information,but also show earlier response to therapy than the change of tumor volume.