视网膜色素上皮细胞间通讯功能激光损伤的防护

Protect intercellular communication of pigment epithelium after laser photocoagulation

目的观察在激光照射下，不同浓度的地塞米松及维拉帕米（ｖｅｒａｐａｍｉｌ）对视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉａｌ，ＲＰＥ）细胞间通讯功能的保护作用。方法取１０只猪眼球分离其ＲＰＥ细胞行体外细胞培养，在激光照射前２ｈ，采用不同浓度的地塞米松及维拉帕米分别孵育其第４代传代细胞，然后测定激光照射后光凝斑周围细胞间通讯功能。结果１２５ｍｇ／Ｌ的地塞米松及２０ｍｇ／Ｌ的维拉帕米均能在激光照射的情况下保护ＲＰＥ细胞间通讯功能，而较高浓度的药物保护作用更大。结论地塞米松和维拉帕米均能在离体条件下保护激光损伤后的ＲＰＥ细胞间通讯功能，且其保护作用与用药浓度有关。

Objective To observe the protective effect on the intercellular communication of retinal pigment epithelial (RPE) cells of dexamethasone (Dex) and verapamil (Ver) at different concentrations after laser photocoagulation. Method Ten pig eyes were used to culture pig pigment epithelium cells. Use different concentrations of Dex and Ver to incubate cultured RPE cells 2 hours before laser coagulation, and then measure the intercellular communication function of confluent cells surrounding the coagulation spots after photocoagulation. Result 125 mg/L Dex and 20 mg/L Ver could protect the intercellular communication of RPE cells under laser photocoagulation. The high concentration of drugs had better result. Conclusion Both Dex and Ver can preserve the intercellular communication in vitro. The protective capacity is related to the concentration of drug.