摘要
目的:观察低强度中频超声对人卵巢癌细胞的杀伤作用。方法:取对数生长期的HO8910细胞,分成实验组和假辐照组,以低强度中频超声(频率1.7MHz)辐照。用MTT法计算细胞抑制率;光镜观察细胞形态,Hoechst33258荧光染色法检测凋亡细胞。结果:低强度中频超声照射各组间细胞抑制率与对照组相比有统计学差异,光镜下可见细胞形态学改变,同时Hoechst33258荧光染色法观察到亮蓝凝聚的凋亡细胞。结论:低强度中频超声对人卵巢癌HO8910细胞体外生长有明显杀伤作用,并可诱导其凋亡。
Objectives： The present study aims to investigate the cytatoxicity of low - intensity ultrasound on ovarian cancer cells. Metheds： H08910 cells were randomly divided into sham radiation group and experimental group. The cells in the experimental group were exposed to ultrasound wave （ 1.7MHz）. The cytotoxicity was measured by MTF assay. The morphological changes of HO8910 cells were observed under light microscope. Apoptosis was analyzed using fluorescent microscopy with Hcechst 33258 stainin. Results： The cytotoxicity was significant difference between experimental group and sham radiation group. Light microscope confirmed the above results, Apoptosis were found by Hoechst 33258 staining method. Conclusion： Low- intensity ultrasound dan significantly kill ovarian cancer cell and induce apoptosis in vitro.
出处
《激光杂志》
CAS
CSCD
北大核心
2011年第1期71-71,73,共2页
Laser Journal
基金
国家自然科学基金资助(30973168)
