摘要
为建立一种快速、简便的O1群霍乱弧菌检测方法,用抗O1群霍乱弧菌多价单克隆抗体致敏胶乳,建立起胶乳凝集试验(LAT),检测粪便标本6h培养物中的霍乱弧菌。LAT检测的敏感性为5×105CFU/ml,检测过程小于5min。在92例临床标本中,44例培养阳性者LAT法均阳性,48例培养阴性者中,有1例假阳性。
To develope a simple and rapid method for detecting V.cholerae O1,latex partices were sensitived with anti V.cholerae O1 monoclonal antibodies,and a latex agglutination test(LAT)detcting V.cholerae O1 in 6 h fecal enrichmen cultures was developed.The detection limit of LAT was 5×10 5 CFU/ml,and detect time was less than 5 min.OF 92 faecal sample from clinically suspected cases of cholera,44 were identified as V.cholerae O1 by culture methods and also gave positive results in the LAT;48 were negative by culture methods but 1 gave false positive in the LAT.This false positive one can be identified by the microsopy.
出处
《临床检验杂志》
CAS
CSCD
北大核心
1999年第3期138-139,共2页
Chinese Journal of Clinical Laboratory Science