苦豆子总碱对豚鼠离体胆囊平滑肌条收缩功能的影响

Effect of Sophora Alopecuroides on Contraction of Isolated Cholecyst Smooth Muscle Strips in Guinea Pigs in Vitro

目的观察苦豆子总碱对豚鼠离体胆囊平滑肌运动功能的影响并初步探讨其作用机制。方法将28只豚鼠随机分为7组,每只豚鼠胆囊制备3条肌条,共84条。将12条胆囊肌条置于灌流肌槽内,采用累积加药方式,苦豆子总碱终浓度分别为1.00×10-5、1.10×10-4和1.10×10-3mmol.L-1。按随机法将其余72条胆囊肌条分为6组:阿托品+苦豆子总碱组、酚妥拉明+苦豆子总碱组、异搏定+苦豆子总碱组、苯海拉明+苦豆子总碱组、消炎痛+苦豆子总碱组、六烃季铵+苦豆子总碱组,每组12条。分别加入拮抗剂阿托品10-6mmol.L-1、酚妥拉明10-6mmol.L-1、异搏定10-7mmol.L-1、苯海拉明10-6mmol.L-1、消炎痛10-6mmol.L-1、六烃季铵10-5mmol.L-1后2m in再依次加入前述累积加药苦豆子总碱溶液。通过FT-100生物张力传感器将信号输出BL-420E+生物机能实验系统记录标本的张力、收缩波平均振幅及收缩频率的变化值。结果苦豆子总碱1.10×10-4和1.10×10-3mmol.L-1可使胆囊平滑肌肌条张力增高(P<0.01);异搏定(10-7mmol.L-1)能明显降低苦豆子总碱(1.10×10-4和1.10×10-3mmol.L-1)对豚鼠胆囊平滑肌条收缩张力的影响(P<0.01)。表明苦豆子总碱拮抗异搏定抑制钙离子的内流。结论苦豆子总碱可提高胆囊肌条的张力,这种作用可能与Ca2+通道有关。

Objective To observe effect and mechanism of sophora alopeeuroides on isolated cho/ecyst smoom muscle strips in guinea pigs in vitro. Methods 28 guinea pig were randomly divided into 7 group. 3 muscular strips of smooth muscle were established in each cholecyst with 84 in total. 12 muscle strips were put in perfused muscle chamber. Using the cumulative administration, Sophora alopecuroides final concentration were 1.00×10^-5 ,1.10×10^-4 and 1.10×10^-3 mmol · L^-1 respectively. 72 muscular strips were randomly divided into 6 groups： atropine ＋ sophora alopeeuroides groups, phenolamine ＋ sophora alopecuroides groups, verapamil ＋ sophora alopeeuroides groups, diphenhydramine ＋ sophora alopecuroides groups, indomethacin ＋ sophora alopecuroides groups, Hexamethonium ＋ sophora alopeeuroides groups, with 12 strips in each group. Musclar strips in the above groups were treated with atropine 10^-6mmol· L^-1, Phenolamine 10^-6mmol· L^-1 verapamil 10^-7 mmol· L^-1, diphenhydramine 10^-6 mmol· L^-1,indomethacin 10^-6 mmol· L^-1, hexamethonum 10^-5 mmol· L^-1 respectively and Sophora alopecuroides at above concentration was added 2 minutes later. Signals were output with FT - 100 biological tension sensor ; tension of samples, average amplitude of systolic wave and changes of systolic frequency were recorded with BL- 420E ＋ biological function experimental system. Results The tension of gallbladder smooth muscle strips was significantly increased in sophora alopecuroide （1.10×10^-4 and 1.10×10^-3 mmol· L^-1）group （P 〈 0. 01 ） ; Verapamil（ 10^-7 mmol· L^-1） could significantly reduce the effect of contraction tention of the sophora alopecuroide （ 1.10×10^-4 and 1.10×10^-3 mmol· L^-1） on cholecyst smooth muscle strips of guinea pig（P 〈 0. 01 ）. Conclusion Sophora alopecuroides can increase the tension of gallbladder muscle strips. Effect and mechanism of sophora alopecuroides on the gallbladder muscle strips may be related to Ca^2＋ channel.